LOCUS       sequence-66245-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Yang CF, Chiang MC, Gray DC, Prabhakaran M, Alvarado M, Juntti SA, 
            Unger EK, Wells JA, Shah NM
  TITLE     Sexually dimorphic neurons in the ventromedial hypothalamus govern 
            mating in both sexes and aggression in males.
  JOURNAL   Cell. 2013 May 9;153(4):896-909. doi: 10.1016/j.cell.2013.04.017.
  PUBMED    23663785
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.04"; journalName: "Cell"; date: "2013-05-9- 9";
            volume: "153"; issue: "4"; pages: "896-909"
COMMENT     Sequence Label: pLenti-lxlplap
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      150..159
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(162..195)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             204..584
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MAPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYK
                     VLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLL
                     PGELAKHAVSEGTKAVTKYTSAK"
     regulatory      597..606
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(648..669)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta
       61 gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag acgaagcttg
      121 ggctgcaggt cgactctagt accggtgccg ccaccatggg tataacttcg tatagcatac
      181 attatacgaa gttatggcgc gccatggcac cagagccagc gaagtctgct cccgccccga
      241 aaaagggctc caagaaggcg gtgactaagg cgcagaagaa aggcggcaag aagcgcaagc
      301 gcagccgcaa ggagagctat tccatctatg tgtacaaggt tctgaagcag gtccaccctg
      361 acaccggcat ttcgtccaag gccatgggca tcatgaattc gtttgtgaac gacattttcg
      421 agcgcatcgc aggtgaggct tcccgcctgg cgcattacaa caagcgctcg accatcacct
      481 ccagggagat ccagacggcc gtgcgcctgc tgctgcctgg ggagttggcc aagcacgccg
      541 tgtccgaggg tactaaggcc gtcaccaagt acaccagcgc taaggatcca ccggtcgcca
      601 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      661 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      721 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      781 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
      841 agcagcacga cttcttcaag tccgccatg
//
