LOCUS       sequence-66247-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Kebache S, Zuo D, Chevet E, Larose L
  TITLE     Modulation of protein translation by Nck-1.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5406-11.
  PUBMED    11959995
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2002-04-16"; volume: "99(8)"; pages: 
            "5406-11"
COMMENT     Sequence Label: pSV HA eIF2beta (Pro110-113)
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     95..114
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        95..113
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             164..190
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ctctggctaa
       61 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
      121 aagctggcta gcgtttaaac ttaagcttac tagtggtacc atgtatgatg ttcctgatta
      181 tgctagcctc ggatccatgt ccggggacga gatgattttt gatcctacta tgagcaaaaa
      241 gaaaaagaag aagaagaagc cttttatgtt agatgaagaa ggtgatgccc agacagaaga
      301 aacccagccc tcagagacaa aagaagtgga gccagaacca actgaagaaa aagacgtgga
      361 cgctgatgag gaagacagta ggaagaaaga tgcttctgat gacttagatg atttgaactt
      421 ctttaatcaa aagaaaaaga agaaaaagac aaaaaagata tttgacattg atgaagctga
      481 agaagctata aaggatgtta agattgagag tgatgctcaa gaggcagccg aggcagagga
      541 tgatctagac attatgcttg gcaacaaaaa aaagaaaaag aagaatgtta aattcccaga
      601 agaagatgaa atactagaaa aagacgaagc tttagaagat gaagacagta aaaaagatga
      661 tggtatttca ttcagtagcc aaactgcttg ggcaggctca gaaagagact acacatatga
      721 ggagttgctg aaccgagtgt tcaacatcat gagagaaaag aatccagata tggttgctgg
      781 agagaagagg aaatttgtta tgaaacctcc acaggtcgtc cgagtaggaa ccaagaaaac
      841 ttcttttgtc aattttacag atatctgtaa actattacat cgtcaaccca aacatctcct
      901 tgcattttta ttggcagaat tgggtacaag tggt
//
