LOCUS       sequence-66254-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Bar-Peled L, Chantranupong L, Cherniack AD, Chen WW, Ottina KA, 
            Grabiner BC, Spear ED, Carter SL, Meyerson M, Sabatini DM
  TITLE     A Tumor suppressor complex with GAP activity for the Rag GTPases 
            that signal amino acid sufficiency to mTORC1.
  JOURNAL   Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044.
  PUBMED    23723238
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2013-05-31- 31"; volume: "340"; 
            issue: "6136"; pages: "1100-6"
COMMENT     Sequence Label: Flag Nprl2 pLJM1
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      54..63
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             63..86
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtgccacca
       61 tggactacaa ggatgacgat gacaagggtg gtggtggtgg tgcgtcgacg atgggcagcg
      121 gctgccgcat cgaatgcata ttcttcagcg agttccaccc cacgctggga cccaagatca
      181 cctatcaggt ccctgaagac ttcatctccc gagagctgtt tgacacagtc caagtgtaca
      241 tcatcaccaa gccagagctg cagaacaagc ttatcactgt cacagctatg gaaaagaagc
      301 tgatcggctg tcctgtgtgc atcgaacaca agaagtacag ccgcaatgct ctcctcttca
      361 acctgggctt cgtgtgtgat gcccaggcca agacctgcgc cctcgagccc attgttaaaa
      421 agctggctgg ctatctgacc acactagagc tagagagcag cttcgtgtcc atggaggaga
      481 gcaagcagaa gttggtgccc atcatgacca tcttgctgga ggagctaaat gcctcaggcc
      541 ggtgcactct gcccattgat gagtccaaca ccatccactt gaaggtgatt gagcagcggc
      601 cagaccctcc ggtggcccag gagtatgatg tacctgtctt taccaaagac aaggaggatt
      661 tcttcaactc acagtgggac ctcactacac aacaaatcct gccctacatt gatgggttcc
      721 gccacatcca gaagatttca gcagaggcag atgtggagct caacctggtg cgcattgcta
      781 tccagaacct gctgtactac ggcgttgtga cactggtgtc catcctccag tactccaatg
      841 tatactgccc aacgcccaag gtccaggacc tggtagatga caagtccctg caagaggcat
      901 gtctatccta c
//
