LOCUS       sequence-66261-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Kebache S, Zuo D, Chevet E, Larose L
  TITLE     Modulation of protein translation by Nck-1.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5406-11.
  PUBMED    11959995
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2002-04-16"; volume: "99(8)"; pages: 
            "5406-11"
COMMENT     Sequence Label: pEGFP eIF2beta C-term (aa 89-331)
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(842..893)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(871..891)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtggatc cccgttagct ttggcacgga
       61 gctgtgctcg cttgtctgtg acagcctgga agccagtttt gatactggca acagagcatc
      121 gagaatgaca agtttcacat tgtaagaaat agagtcgggt gtccttctgt aggattgtgt
      181 ccggtgaccg gcatgtgtga caagtgacat attccttgat atatcttctc aagacatttt
      241 ctatttgttt ctgttggaat cttcctttga ttacaagttg gttattacca tctatagaac
      301 cacttgtacc caattctgcc aataaaaatg caaggagatg tttgggttga cgatgtaata
      361 gtttacagat atctgtaaaa ttgacaaaag aagttttctt ggttcctact cggacgacct
      421 gtggaggttt cataacaaat ttcctcttct ctccagcaac catatctgga ttcttttctc
      481 tcatgatgtt gaacactcgg ttcagcaact cctcatatgt gtagtctctt tctgagcctg
      541 cccaagcagt ttggctactg aatgaaatac catcatcttt tttactgtct tcatcttcta
      601 aagcttcgtc tttttctagt atttcatctt cttctgggaa tttaacattc ttctttttct
      661 tttttttgtt gccaagcata atgtcaaggt catcctctgg ctcggctggc tcttgagcat
      721 cactctcaat cttaacatcc tttatagctt cttcagcttc atcaatgtca aacatgaatt
      781 cgaagcttga gctcgagatc tgagtccggt agcgctagcg gatctgacgg ttcactaaac
      841 cagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
      901 cggagttgtt acgacattgg aagtccgttg atttgt
//
