LOCUS       sequence-66297-e         674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 674)
  TITLE     Reya 2009
REFERENCE   2  (bases 1 to 674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MSCV-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(169..585)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(185..201)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(217..239)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 ctcgtcaaga agacagggcc aggtttccgg gccctcacat tgccaaaaga cggcaatatg
       61 gtggaaaata acatatagac aaacgcacac cggccttatt ccaagcggct tcggccagta
      121 acgttagggg gggggcggaa ttgatcccgc tcgaggttaa cgaattccgg cgcctagaga
      181 aggagtgagg gctggataaa gggaggatcg aggcggggtc gaacgaggag gttcaagggg
      241 gagagacggg gcggatggag gaagaggagg cggaggctta aggtgtacaa agggcttgac
      301 ccagggaggg gggtcaaaag ccaaggcttc ccaggtcacg atgtagggga cctggtctgg
      361 gtgtccatgc gggccaggtg aaaagacctt gatcttaacc tgggtgatga ggtctcggtt
      421 aaaggtgccg tctcgcggcc atccgacgtt aaaggttggc cattctgcag agcagaaggt
      481 aacccaacgt ctcttcttga catctaccga ctggttgtga gcgatccgct cgacatcttt
      541 ccagtgacct aaggtcaaac ttaagggagt ggtaacagtc tggccctaat tttcagacaa
      601 atacagaaac acagtcagac agagacaaca cagaacgatg ctgcagcgct gcagcagaca
      661 agacgcgcgg cttc
//
