LOCUS       sequence-66308-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Marx CJ, Lidstrom ME
  TITLE     Broad-host-range cre-lox system for antibiotic marker recycling in 
            gram-negative bacteria.
  JOURNAL   Biotechniques. 2002 Nov;33(5):1062-7.
  PUBMED    12449384
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques."; date: "2002-11"; volume: "33(5)"; pages: "1062-7"
COMMENT     Sequence Label: pCM351
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(201..305)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    complement(389..422)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(442..447,448..464,465..470)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
       61 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
      121 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
      181 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
      241 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
      301 ccgcgcacat ttccccgaaa agtgccacct gacgtctaga tctgaattca gctgtacaat
      361 tggtaccatg gatgcatatg gcggccgcat aacttcgtat agcatacatt atacgaagtt
      421 atctacgcgt caattctcga attgacataa gcctgttcgg ttcgtaaact gtaatgcaag
      481 tagcgtatgc gctcacgcag ctggtccaga accttgaccg aacgcagcgg tggtaacggc
      541 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg tacagtctat gcctcgggca
      601 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
      661 gttacgcagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaggtgg
      721 ctcaagtatg ggcatcattc gcacatgtag gctcggccct gaccaagtca aatccatgcg
      781 ggctgctctt gatcttttcg gtcgtgagtt cggagacgta gccacctact cccaacatca
      841 gccggactcc gattacctcg ggaacttgct ccgtagtaag acattcatcg cgcttgctgc
//
