LOCUS       sequence-66309-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Marx CJ, Lidstrom ME
  TITLE     Broad-host-range cre-lox system for antibiotic marker recycling in 
            gram-negative bacteria.
  JOURNAL   Biotechniques. 2002 Nov;33(5):1062-7.
  PUBMED    12449384
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques."; date: "2002-11"; volume: "33(5)"; pages: "1062-7"
COMMENT     Sequence Label: pCM351
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(106..139)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tacaagttgg gcatacggga agaagtgatg cactttgata tcgacccaag taccgccacc
       61 taacaattcg ttcaagccga gatcggcttc ccggccgacg cgtagataac ttcgtatagc
      121 atacattata cgaagttatg gatccagctt atcgataccg cgggccctac gtacgcgtgt
      181 taaccggtga gctcactaga ggatccagcc gaccaggctt tccacgcccg cgtgccgctc
      241 catgtcgttc gcgcggttct cggaaacgcg ctgccgcgtt tcgtgattgt cacgctcaag
      301 cccgtagtcc cgttcgagcg tcgcgcagag gtcagcgagg gcgcggtagg cccgatacgg
      361 ctcatggatg gtgtttcggg tcgggtgaat cttgttgatg gcgatatgga tgtgcaggtt
      421 gtcggtgtcg tgatgcacgg cactgacgcg ctgatgctcg gcgaagccaa gcccagcgca
      481 gatgcggtcc tcaatcgcgc gcaacgtctc cgcgtcgggc ttctctcccg cgcggaagct
      541 aaccagcagg tgataggtct tgtcggcctc ggaacgggtg ttgccgtgct gggtcgccat
      601 cacctcggcc atgacagcgg gcagggtgtt tgcctcgcag ttcgtgacgc gcacgtgacc
      661 caggcgctcg gtcttgcctt gctcgtcggt gatgtacttc accagctccg cgaagtcgct
      721 cttcttgatg gagcgcatgg ggacgtgctt ggcaatcacg cgcacccccc ggccgtttta
      781 gcggctaaaa aagtcatggc tctgccctcg ggcggaccac gcccatcatg accttgccaa
      841 gctcgtcctg cttctcttcg atcttcgcca gcagggcgag gatcgtggca tcaccgaacc
      901 gcgccgtgcg cgggtcgtcg gtgagccaga
//
