LOCUS       sequence-66312-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector. Inserted genes are 
            expressed only in the resence of rtTA3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  TITLE     other plasmids
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti CMVTRE3G Puro DEST (w811-1)
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(102..108,109..126,127..132))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(157..281)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(327..705)
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     primer_bind     complement(394..418)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    463..481
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    499..517
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    535..553
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    571..589
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    643..661
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    679..697
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    732..849
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
       61 agctcctgaa aatctcgacg gatcctaact caaaatccac acattatacg agccggaagc
      121 ataaagtgta aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt
      181 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
      241 tcattttacg tttctcgttc agcttttttg tacaaacttg ttgatatctg cagaattcca
      301 ccacactgga ctagtcgacg aaccggttta cgagggtagg aagtggtacg gaaagttggt
      361 ataagacaaa agtgttgtgg aattgctcca ggcgatctga cggttcacta aacgagctct
      421 gcttttatag gcgcccaccg tacacgccta aagcttatac gttctctatc actgataggg
      481 agtaaactgg atatacgttc tctatcactg atagggagta aactgtagat acgttctcta
      541 tcactgatag ggagtaaact ggtcatacgt tctctatcac tgatagggag taaactcctt
      601 atacgttctc tatcactgat agggagtaaa gtctgcatac gttctctatc actgataggg
      661 agtaaactct tcatacgttc tctatcactg atagggagta aagccaactc gaggctagtc
      721 tcgtgatcga taaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg
      781 tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt
      841 cttttaaaag ttaaccgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg
      901 tctctctctc
//
