LOCUS       sequence-66313-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector. Inserted genes are 
            expressed only in the resence of rtTA3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  TITLE     other plasmids
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti CMVTRE3G Puro DEST (w811-1)
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(126..250)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    281..869
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(334..354)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(752..763)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
       61 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
      121 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
      181 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt
      241 acaaagtggt tgatatccag cacagtggcg gccgctcgac aatcaacctc tggattacaa
      301 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
      361 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
      421 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
      481 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
      541 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
      601 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
      661 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
      721 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
      781 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
      841 tcggatctcc ctttgggccg cctccccgcc tggaatt
//
