LOCUS       sequence-66317-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Lu J, Beatty L, Pinthus J
  TITLE     Dual expression recombinase based (DERB) single vector system for 
            high throughput screening and verification of protein interactions 
            in living cells
  JOURNAL   Nat Prec (2008).
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Prec 
            (2008)."
COMMENT     Sequence Label: E pCMV-CFPLoxP-YFPatt
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(133..257)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(318..337)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(319..337)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    474..595
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(511..530)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     565..584
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(739..760)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
       61 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
      121 ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
      181 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
      241 ttcttgtaca aagtggttga tgggcggccg ctctagaggg cccaagctta cgcgtgcatg
      301 cgacctcata gctctctccc tatagtgagt cgtattataa gctaggcact ggccgtacta
      361 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
      421 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
      481 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
      541 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg
      601 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
      661 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
      721 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
      781 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
      841 taatcaagtt ttttggggtc
//
