LOCUS       sequence-66318-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Lu J, Beatty L, Pinthus J
  TITLE     Dual expression recombinase based (DERB) single vector system for 
            high throughput screening and verification of protein interactions 
            in living cells
  JOURNAL   Nat Prec (2008).
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Prec 
            (2008)."
COMMENT     Sequence Label: Y pFe-YcLoxP-YnAtt
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    123..156
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(169..190)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtggatcgag cgtggagcca aacgcagtac aaagtgttac ccctctagac ctggaaagac
       61 caggcggagt ttcaagttgc acgttttgtg tcattgggga aacctgctcc tagggtcgac
      121 ctataacttc gtataatgta tgctatacga agttatcccg gcggcggtca cgaactccag
      181 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact ggtagctcag
      241 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      301 gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat
      361 gccgttcttc tgcttgtcgg ccatgatata tctccttcac cggttgcttt gaattagcgg
      421 tggttttcac aacacctaaa aaagggttta aaagatacct ttgaaccgct aagaagcccg
      481 agaattagct ccgctcaaaa ctcaagggga caaattccaa aaatgacttc cagcgccagg
      541 ctggcctgac tagtctccac ccaccaaatg tgaacaaact ccaacgccat tacatcccct
      601 ccccccgccg cgactagccg tgctcaaaag cccgaggtga ctattgcggc cgataggacc
      661 acggggtcac aggaagcagc agccggtgag ggaccaggcc ctcttccttt gtgtgggtga
      721 ctcacccgcc cgctccaccg ggctgccgcg tcctccattt tgagctcctt gcaacagggc
      781 ccgggagcgg ccatctttcc acgcacgcaa ctggtgccgg acgggatggc ctcaccctag
      841 ttagggaggc agggcaacgc
//
