LOCUS       sequence-66319-e       13078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  luciferase reporter for BmalI promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13078)
  AUTHORS   Brown SA, Fleury-Olela F, Nagoshi E, Hauser C, Juge C, Meier CA, 
            Chicheportiche R, Dayer JM, Albrecht U, Schibler U
  TITLE     The period length of fibroblast circadian gene expression varies 
            widely among human individuals.
  JOURNAL   PLoS Biol. 2005 Oct;3(10):e338. Epub 2005 Sep 27.
  PUBMED    16167846
REFERENCE   2  (bases 1 to 13078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2005-10"; volume: "3(10)"; pages: "e338. Epub 2005 
            Sep 27"
COMMENT     Sequence Label: pABpuro-BluF
FEATURES             Location/Qualifiers
     source          1..13078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    678..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1298..1531
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1716..1760
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     regulatory      3170..3179
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3176..4828
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3242..3260)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        5955..6284
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6135..6270
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6294..6893
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(6294..6313)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6790..6810
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6959..7547
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7012..7032)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7430..7441)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7638..7871
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7896..7914)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7897..7914)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8204..8223)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8438..8457)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8617..8635)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8703..8807
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8808..8876,8877..9668)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9026..9045)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9839..10427
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10328..10347
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10581..10598
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        10673..11002
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10853..10988
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12136..12201
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12331..12351
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12776..12910
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12813..12832)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12867..12886
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacctgaaag
      661 cgaaagggaa accagagctc tctcgacgca ggactcggct tgctgaagcg cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     1201 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     1261 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     1321 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     1381 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     1441 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     1501 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     1561 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     1621 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     1681 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     1741 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     1801 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     1861 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     1921 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     1981 acagatccat tcgattagtg aacggatctc gacggtatcg gatctcgagc tcaagcttcg
     2041 aattcgccct tgggctacaa cagaacaact aaccacccaa acaaacaact ttgcaacttt
     2101 tctttctttc ccgctccttt gatacttttg caaagtacat ttgctagggc catgtccata
     2161 acatgtaata gaatcttgct caggtctcac cctgtggccc cggctagctc agtactcgcg
     2221 attatgcccc tgcctcagct tcttgagggt tggaattaca gactacgcca ccacccccgg
     2281 gagacttctc ttccgatcgg gaatgacagc tccactggac gcttggaggt caagagaaaa
     2341 aaaaaaaaaa aaaaaaaaag aacgcgcgat ccggttagtc gtcggaagga aaagtcttct
     2401 aaatgcgcta gctattagcg ctgtggttcc gactaggaca agccaggggt ttcaggaagg
     2461 ttctgtcact ctgtgttcct aaaatgtggt ttcctagatg gagctggaga gggatgggcg
     2521 aagagatgcg gcgttttctc agtgttctgc gagccaggaa ggggacggag gaggagggag
     2581 agctgagagg cagtcactag ccagggacgc ggctgaggag caggggacaa cggcgagctc
     2641 gcagagtccg caacgcagtg gcctcagcga gctttagacc tgagggggaa aaccgagggc
     2701 tggggcaaga aatccacaga gcgtgccaat tggtccactc ctcggggcgt gtgcttctgt
     2761 gcgccaaatg attggtgaag ggaaagtagc aggtaaacca gccctgccgt cttgccattg
     2821 gtcagaggct ttcctatcgg tcactcgatt ggctagccta acgcagagca gaacgcgaat
     2881 tggtttgggt tgtccgccaa gacaactccg ttcgctctct ctgattggct aacgggaaga
     2941 ggcaggtatc cgggctgcgc ggctcctcca ttggtgggcg gggaaggggg gttgggcaca
     3001 gcgattggtg ggcggggggc cgggcctggg ccggcgggga gcggattggt cggaaagtag
     3061 gttagtggtg cgacatttag ggaaggcaga aagtaggtca gggacggagg tgcctgttta
     3121 cccgcgccgg acttgccgcc gccgccgccg cgggatcgat ccaccggtcg ccaccatgga
     3181 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctagagg atggaaccgc
     3241 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt
     3301 tacagatgca catatcgagg tgaacatcac gtacgcggaa tacttcgaaa tgtccgttcg
     3361 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag
     3421 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt
     3481 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatga acatttcgca
     3541 gcctaccgta gtgtttgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa
     3601 attaccaata atccagaaaa ttattatcat ggattctaaa acggattacc agggatttca
     3661 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt
     3721 accagagtcc tttgatcgtg acaaaacaat tgcactgata atgaactcct ctggatctac
     3781 tgggttacct aagggtgtgg cccttccgca tagaactgcc tgcgtcagat tctcgcatgc
     3841 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc
     3901 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt
     3961 cgtcttaatg tatagatttg aagaagagct gtttttacga tcccttcagg attacaaaat
     4021 tcaaagtgcg ttgctagtac caaccctatt ttcattcttc gccaaaagca ctctgattga
     4081 caaatacgat ttatctaatt tacacgaaat tgcttctggg ggcgcacctc tttcgaaaga
     4141 agtcggggaa gcggttgcaa aacgcttcca tcttccaggg atacgacaag gatatgggct
     4201 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt
     4261 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct
     4321 gggcgttaat cagagaggcg aattatgtgt cagaggacct atgattatgt ccggttatgt
     4381 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga
     4441 catagcttac tgggacgaag acgaacactt cttcatagtt gaccgcttga agtctttaat
     4501 taaatacaaa ggataccagg tggcccccgc tgaattggag tcgatattgt tacaacaccc
     4561 caacatcttc gacgcgggcg tggcaggtct tcccgacgat gacgccggtg aacttcccgc
     4621 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt
     4681 cgccagtcaa gtaacaaccg ccaaaaagtt gcgcggagga gttgtgtttg tggacgaagt
     4741 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc
     4801 caagaagggc ggaaagtcca aattgtaagc ggccgccagt gtgatggata tctgcagaat
     4861 tcgcccttta gacactacat ttgctttggc aacagctgca gtatcaaagt gcattaatgg
     4921 tgaagtttta cagtctgtga agcttactgg atagagagag aacagctttt atgtactgac
     4981 tccataaaag ccacctcaga gccattgata caagtcaatc taccatgtgt aacttcagac
     5041 aaagtggaac taaacctgct ccagtgtttc ctcatcattg agtttgggct agctgtggat
     5101 agcttgcatt aattgtatat tttggatttt gtttgtgttg aattttttaa tcattgtgca
     5161 cagaagcatc attggtagct tttatatgca aaatggtcat ttcagatgta tggtgttttt
     5221 acactacaaa gaagtccccc atgtggatat ttcttatact aattgtatca taaagctgtt
     5281 tattcttcct tgtaagaatc ctttactata aatatgggtt aaagtataat gtattagaca
     5341 gttaaatatt tttaataaat gtttcccttg ttctataagt actgttccca attgataatt
     5401 aggaagaaaa tcttcatatg ctacagggct ggttcatcca cttcaccagc ggtctaaaca
     5461 cattcctgct gttatgttta caatgtcctt ttgctcttat aatgacttct tccatgctac
     5521 cagcttagac atattataaa aatagctcac ttccaaactg tagccacctc aacttatatg
     5581 aaaacaaaga tatcaactcc aatttccagt gcttagattt caagattaca agctggcttc
     5641 tctagacctg cagttacatg gtgtgctcaa gtctaccagg ggtagatgta agcgacggca
     5701 tctgctctct ttctatggtc ccatcgacaa actgagatgt gatcccacca aactaaagtt
     5761 cactctggaa accaatgggt tggtttactg ggcttccata cagacacagg cgagaggttc
     5821 cctacaggta tgctcctact caaaggccac actagaaagc ctttactggg gctggaaggg
     5881 cgaattccag cacactggcg gccgttacta gtggatccga attcgccagc acagtggtcg
     5941 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     6001 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     6061 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     6121 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6181 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     6241 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt accatgaccg
     6301 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     6361 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     6421 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     6481 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     6541 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     6601 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     6661 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     6721 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     6781 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     6841 agtgcccgaa ggaccgcgcg acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
     6901 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc catcgaatta attccgataa
     6961 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     7021 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     7081 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     7141 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     7201 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     7261 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     7321 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     7381 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     7441 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     7501 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gggaattaac
     7561 gagctcggta cctttaaaac caatgactta caaggcagct gtaaatctta gccacttttt
     7621 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaaacaaa atctgctttt
     7681 tgcttgtact gggtctctct ggttagacca aatctgagcc tgggagctct ctggctaact
     7741 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7801 ccgtctgttg tgtgactctg gtaactagag atccctcaaa cccttttagt cagtgtggaa
     7861 aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg
     7921 tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag
     7981 cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc
     8041 gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg
     8101 gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc
     8161 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     8221 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     8281 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     8341 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     8401 tcgcataagg gagagcgtcg atatggtgca ctctcagtac aatctgctct gatgccgcat
     8461 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     8521 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     8581 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     8641 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     8701 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8761 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     8821 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8881 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8941 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     9001 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     9061 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     9121 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     9181 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     9241 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     9301 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     9361 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     9421 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     9481 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     9541 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     9601 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     9661 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9721 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9781 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9841 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9901 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9961 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
    10021 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    10081 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    10141 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    10201 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    10261 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    10321 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    10381 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    10441 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    10501 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    10561 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    10621 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca
    10681 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
    10741 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    10801 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
    10861 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    10921 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
    10981 tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga
    11041 gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg
    11101 aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga
    11161 acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg
    11221 tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg
    11281 aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc
    11341 gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa
    11401 ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat
    11461 tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc
    11521 gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt
    11581 tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc
    11641 gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa
    11701 cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg
    11761 caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag
    11821 tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca
    11881 tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac
    11941 gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg
    12001 atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg
    12061 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
    12121 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
    12181 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
    12241 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
    12301 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
    12361 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
    12421 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
    12481 atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca taacatactg
    12541 ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg
    12601 tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc
    12661 ttgactagag atcataatca gccataccac atttgtagag cttttacttg ctttaaaaaa
    12721 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
    12781 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    12841 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    12901 tgtgtggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
    12961 ccctattacc actgccaaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
    13021 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//
