LOCUS       sequence-66320-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Lu J, Beatty L, Pinthus J
  TITLE     Dual expression recombinase based (DERB) single vector system for 
            high throughput screening and verification of protein interactions 
            in living cells
  JOURNAL   Nat Prec (2008).
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Prec 
            (2008)."
COMMENT     Sequence Label: Y pFe-YcLoxP-YnAtt
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(92..94,95..97,98..556)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(137..158)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(398..417)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    569..693
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(718..723,724..741,742..748)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(868..887)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
       61 agaggatccc taccggtgaa ggagatatac catggtgagc aagggcgagg agctgttcac
      121 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      181 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      241 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca
      301 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      361 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      421 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      481 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
      541 cgtctatatc atggcctcga gcccatcaac aagtttgtac aaaaaagctg aacgagaaac
      601 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata
      661 atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac ctcaggcttt
      721 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt
      781 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
      841 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
      901 aaccagaccg ttcagctgga tattacggcc ttt
//
