LOCUS       sequence-66323-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Shen MW, Fang F, Sandmeyer S, Da Silva NA
  TITLE     Development and characterization of a vector set with regulated 
            promoters for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):495-503. doi: 10.1002/yea.2930. Epub 2012 Nov
            19.
  PUBMED    23166051
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2930"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "495-503"
COMMENT     Sequence Label: pXP731
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    581..614
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cacctaacaa aacggcatca gccttcttgg aggcttccag cgcctcatct ggaagtggga
       61 cacctgtagc ttcgatagca gcaccaccaa ttaaatgatt ttcgaaatcg aacttgacat
      121 tggaacgaac atcagaaata gctttaagaa ccttaatggc ttcggctgtg atttcttgac
      181 caacgtggtc acctggcaaa acgacgatct tcttaggggc agacattaga atggtatatc
      241 cttgaaatat atatatatat tgctgaaatg taaaaggtaa gaaaagttag aaagtaagac
      301 gattgctaac cacctattgg aaaaacaata ggtccttaaa taatattgtc aacttcaagt
      361 attgtgatgc aagcatttag tcatgaacgc ttctctattc tatatgaaaa gccggttccg
      421 gcgctctcac ctttcctttt tctcccaatt tttcagttga aaaaggtata tgcgtcaggc
      481 gacctctgaa attaacaaaa aatttccagt catcgaattt gattctgtgc gatagcgccc
      541 ctgtgtgttc tcgttatgtt gaggaaaaaa ataatggtta ataacttcgt ataatgtatg
      601 ctatacgaag ttatcccggg taccgagctc gaattcgtaa cttacacgcg cctcgtatct
      661 tttaatgatg gaataatttg ggaatttact ctgtgtttat ttatttttat gttttgtatt
      721 tggattttag aaagtaaata aagaaggtag aagagttacg gaatgaagaa aaaaaaataa
      781 acaaaggttt aaaaaatttc aacaaaaagc gtactttaca tatatattta ttagacaaga
      841 aaagc
//
