LOCUS       sequence-66324-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Shen MW, Fang F, Sandmeyer S, Da Silva NA
  TITLE     Development and characterization of a vector set with regulated 
            promoters for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):495-503. doi: 10.1002/yea.2930. Epub 2012 Nov
            19.
  PUBMED    23166051
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2930"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "495-503"
COMMENT     Sequence Label: pXP731
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(2..35)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(81..328)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     294..312
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(397..631)
                     /gene="S. cerevisiae CUP1"
                     /label=CUP1 promoter
                     /note="inducible metallothionein promoter, regulated by 
                     Cu2+"
     primer_bind     complement(680..699)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     799..821
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cataacttcg tatagcatac attatacgaa gttatcccgg ggatcctcta gagtcgacct
       61 gcaggcatgc aagcttggcc gcaaattaaa gccttcgagc gtcccaaaac cttctcaagc
      121 aaggttttca gtataatgtt acatgcgtac acgcgtctgt acagaaaaaa aagaaaaatt
      181 tgaaatataa ataacgttct taatactaac ataactataa aaaaataaat agggacctag
      241 acttcaggtt gtctaactcc ttccttttcg gttagagcgg atgtgggggg agggcgtgaa
      301 tgtaagcgtg acataactaa ttacatgact cgagcctagg tttatgtgat gattgattga
      361 ttgattgtac agtttgtttt tcttaatatc tatttcgatg acttctatat gatattgcac
      421 taacaagaag atattataat gcaattgata caagacaagg agttatttgc ttctctttta
      481 tatgattctg acaatccata ttgcgttggt agtctttttt gctggaacgg ttcagcggaa
      541 aagacgcatc gctctttttg cttctagaag aaatgccagc aaaagaatct cttgacagtg
      601 actgacagca aaaatgtctt tttctaacta gtaacaaggc taagatcata tggaattcct
      661 atgcctggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
      721 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
      781 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
      841 aacgcgcgag acgaaagggc ctcctgatac gcc
//
