LOCUS       sequence-66325-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Shen MW, Fang F, Sandmeyer S, Da Silva NA
  TITLE     Development and characterization of a vector set with regulated 
            promoters for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):495-503. doi: 10.1002/yea.2930. Epub 2012 Nov
            19.
  PUBMED    23166051
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2930"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "495-503"
COMMENT     Sequence Label: pXP811
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    584..617
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cagcacctaa caaaacggca tcagccttct tggaggcttc cagcgcctca tctggaagtg
       61 ggacacctgt agcttcgata gcagcaccac caattaaatg attttcgaaa tcgaacttga
      121 cattggaacg aacatcagaa atagctttaa gaaccttaat ggcttcggct gtgatttctt
      181 gaccaacgtg gtcacctggc aaaacgacga tcttcttagg ggcagacatt agaatggtat
      241 atccttgaaa tatatatata tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa
      301 gacgattgct aaccacctat tggaaaaaca ataggtcctt aaataatatt gtcaacttca
      361 agtattgtga tgcaagcatt tagtcatgaa cgcttctcta ttctatatga aaagccggtt
      421 ccggcgctct cacctttcct ttttctccca atttttcagt tgaaaaaggt atatgcgtca
      481 ggcgacctct gaaattaaca aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg
      541 cccctgtgtg ttctcgttat gttgaggaaa aaaataatgg ttaataactt cgtataatgt
      601 atgctatacg aagttatccc gggtaccgag ctcgaattca acgaagcatc tgtgcttcat
      661 tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc
      721 atttttacag aacagaaatg caacgcgaaa gcgctatttt accaacgaag aatctgtgct
      781 tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga
      841 gctgcatttt tacagaacag aaatgcaacg cgagagcgct attttaccaa caaagaatct
      901 atacttcttt tt
//
