LOCUS       sequence-66327-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Fan B, Malato Y, Calvisi DF, Naqvi S, Razumilava N, Ribback S, Gores
            GJ, Dombrowski F, Evert M, Chen X, Willenbring H
  TITLE     Cholangiocarcinomas can originate from hepatocytes in mice.
  JOURNAL   J Clin Invest. 2012 Aug 1;122(8):2911-5. doi: 10.1172/JCI63212. Epub
            2012 Jul 17.
  PUBMED    22797301
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1172/JCI63212"; 
            journalName: "J Clin Invest"; date: "2012-08-1- 1"; volume: "122"; 
            issue: "8"; pages: "2911-5"
COMMENT     Sequence Label: pT3-EF1a-NICD1
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     16..35
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        16..34
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    113..137
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             174..203
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gggattagct tggtctaata cgactcacta tagggagacc caagctggct aggtaagctt
       61 ggtaccgagc tcggatccac tagtccagtg tggtggaatt ctgcagatat caacaagttt
      121 gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac tggatccacc atggagcaaa
      181 agctcatttc tgaagaggac ttgagatcca ccatgcagca tggccagctc tggttccctg
      241 agggtttcaa agtgtcagag gccagcaaga agaagcggag agagcccctc ggcgaggact
      301 cagtcggcct caagcccctg aagaatgcct cagatggtgc tctgatggac gacaatcaga
      361 acgagtgggg agacgaagac ctggagacca agaagttccg gtttgaggag ccagtagttc
      421 tccctgacct gagtgatcag actgaccaca gacagtggac ccagcagcac ctggacgctg
      481 ctgacctgcg catgtctgcc atggccccaa caccgcctca gggggaggtg gatgctgact
      541 gcatggatgt caatgttcga ggaccagatg gcttcacacc cctcatgatt gcctcctgca
      601 gtggaggggg ccttgagaca ggcaacagtg aagaagaaga agatgcacct gctgtcatct
      661 ctgacttcat ctaccagggc gccagcttgc acaaccagac agaccgcacc ggggagaccg
      721 ccttgcactt ggctgcccga tactctcgtt cagatgctgc aaagcgcttg ctggaggcca
      781 gtgcagatgc caacatccag gacaacatgg gccgtactcc gttacatgca gcagtt
//
