LOCUS       sequence-66329-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Shen MW, Fang F, Sandmeyer S, Da Silva NA
  TITLE     Development and characterization of a vector set with regulated 
            promoters for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):495-503. doi: 10.1002/yea.2930. Epub 2012 Nov
            19.
  PUBMED    23166051
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2930"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "495-503"
COMMENT     Sequence Label: pXP812
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(69..102)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa ttatatcagt
       61 tattacccat aacttcgtat agcatacatt atacgaagtt atcccgggta ccgagctcga
      121 attcaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat
      181 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct
      241 attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg
      301 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga
      361 gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga
      421 gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat
      481 aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact
      541 ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt
      601 actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac
      661 cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg
      721 tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt
      781 tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc
      841 taaagagtaa tactagagat aa
//
