LOCUS       sequence-66331-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Tschaharganeh DF, Chen X, Latzko P, Malz M, Gaida MM, Felix K, Ladu 
            S, Singer S, Pinna F, Gretz N, Sticht C, Tomasi ML, Delogu S, Evert 
            M, Fan B, Ribback S, Jiang L, Brozzetti S, Bergmann F, Dombrowski F,
            Schirmacher P, Calvisi DF, Breuhahn K
  TITLE     Yes-Associated Protein Up-regulates Jagged-1 and Activates the NOTCH
            Pathway in Human Hepatocellular Carcinoma.
  JOURNAL   Gastroenterology. 2013 Jun;144(7):1530-1542.e12. doi: 
            10.1053/j.gastro.2013.02.009. Epub 2013 Feb 16.
  PUBMED    23419361
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1053/j.gastro.2013.02.009"; journalName: "Gastroenterology"; 
            date: "2013-06"; volume: "144"; issue: "7"; pages: "1530-1542.e12"
COMMENT     Sequence Label: pT3-EF1a-YapS127A
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..32
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13..31
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    110..134
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             165..188
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 attgcttggt actaatacga ctcactatag ggagacccaa gctggctagg taagcttggt
       61 accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcaa caagtttgta
      121 caaaaaagca ggctttaaag gaaccaattc agtcgaccac catggactac aaagacgatg
      181 acgataaagc aaggctcgaa tcggtaccta tggatcccgg gcagcagccg ccgcctcaac
      241 cggcccccca gggccaaggg cagccgcctt cgcagccccc gcaggggcag ggcccgccgt
      301 ccggacccgg gcaaccggca cccgcggcga cccaggcggc gccgcaggca ccccccgccg
      361 ggcatcagat cgtgcacgtc cgcggggact cggagaccga cctggaggcg ctcttcaacg
      421 ccgtcatgaa ccccaagacg gccaacgtgc cccagaccgt gcccatgagg ctccggaagc
      481 tgcccgactc cttcttcaag ccgccggagc ccaaatccca ctcccgacag gccagtactg
      541 atgcaggcac tgcaggagcc ctgactccac agcatgttcg agctcatgcc tctccagctt
      601 ctctgcagtt gggagctgtt tctcctggga cactgacccc cactggagta gtctctggcc
      661 cagcagctac acccacagct cagcatcttc gacagtcttc ttttgagata cctgatgatg
      721 tacctctgcc agcaggttgg gagatggcaa agacatcttc tggtcagaga tacttcttaa
      781 atcacatcga tcagacaaca ac
//
