LOCUS       sequence-66333-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Shen MW, Fang F, Sandmeyer S, Da Silva NA
  TITLE     Development and characterization of a vector set with regulated 
            promoters for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):495-503. doi: 10.1002/yea.2930. Epub 2012 Nov
            19.
  PUBMED    23166051
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2930"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "495-503"
COMMENT     Sequence Label: pXP742
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(619..638)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     738..760
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(798..816)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 actcgagact agttgtgtat tacgatatag ttaatagttg atagttgatt gtatgctttt
       61 tgtagcttga tattctattt accaagaaga aacaagaagt gataaaaaca acaagagagc
      121 agtagtaaga gtatttcgag tgtgaaaaaa gtcgctactg gcactctatt tatatgtgat
      181 aggcatgcta tagctttacc aaaaagtgaa ccccatttct atgctctcct ctgccttttt
      241 tttttttttt tttttcattc tctcaatctg aaattctctt atttctccaa cttataagtt
      301 ggagatgccc ggtgttccgg cagaggagat cagtctcgtg aagtggatgg tttcccgcct
      361 gcgggcaaaa cgtcataaca tttttatgag cgaaagccgt taatgaagac aaaatccctt
      421 aattaaaaca ttagaatggt gattagaaag gcaggattaa tcagttacac aggctgtaac
      481 cggagagacg gatcataagg caatttttag ataagactgg ttagagttct tggcatcaga
      541 aaatttgaga aacgattttt ccgtttgttt gcccctacgt tttgcggccg ctaaatcata
      601 tgcctggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
      661 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
      721 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
      781 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
      841 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      901 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctga
//
