LOCUS       sequence-66339-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Tschaharganeh DF, Chen X, Latzko P, Malz M, Gaida MM, Felix K, Ladu 
            S, Singer S, Pinna F, Gretz N, Sticht C, Tomasi ML, Delogu S, Evert 
            M, Fan B, Ribback S, Jiang L, Brozzetti S, Bergmann F, Dombrowski F,
            Schirmacher P, Calvisi DF, Breuhahn K
  TITLE     Yes-Associated Protein Up-regulates Jagged-1 and Activates the NOTCH
            Pathway in Human Hepatocellular Carcinoma.
  JOURNAL   Gastroenterology. 2013 Jun;144(7):1530-1542.e12. doi: 
            10.1053/j.gastro.2013.02.009. Epub 2013 Feb 16.
  PUBMED    23419361
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1053/j.gastro.2013.02.009"; journalName: "Gastroenterology"; 
            date: "2013-06"; volume: "144"; issue: "7"; pages: "1530-1542.e12"
COMMENT     Sequence Label: pT3-EFa1-HA-Jag1
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    29..53
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(80..169)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 cgagcggccg ccactgtgct ggatatcaac cactttgtac aagaaagctg ggtctagata
       61 tctcgagtca gcactgagca gcgtaatctg gaacgtcata tggataggat cctgcatagt
      121 ccgggacgtc atagggatag cccgcatagt caggaacatc gtatgggtaa aagatgcggc
      181 cgccaccgcg gattacgatg tactccatcc ggttcaagct ctgggcactt tccaagtctc
      241 tgttgtcctg tttgtttgtc cagttcgggt gttttgtcgg cgtgccgctg ggggccttct
      301 cctctctgtc taccagcgtg tacacgggct gtttggcaaa ccggactttc tgctggtgct
      361 tatccatatc atcctcttcc acttccgagt tgtgtgtcct gatttttgac attttggagt
      421 ttttgttctc ataatccttg atggggactg tgttggctcc gtgtttctcg atggggtttt
      481 tgatttggtt cagctgttcg cgcacattgt tggtggtgtt gtcctcaggg gcagagtggg
      541 tgtggctgct gggcttccgc cgccgcttcc gcacacacca gtagaaggct gtcaccaagc
      601 aacagaccca agcgactgtt aagacagagc tcagcagagg aaccaggaaa tctgttctgt
      661 ttttcagagg gcgcctctga actctgactt ctgcaacagc agcgataagt gagctgttcc
      721 catcccgttt actaacgaga tctattattt tatcagtaat ttccttgaca gggtttccat
      781 catcccggat gtcttcggca gaaatggcca cgtgtat
//
