LOCUS       sequence-66347-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral Gateway destination vector. Blasticidin 
            selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja 
            R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver 
            SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, 
            Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE
  TITLE     A public genome-scale lentiviral expression library of human ORFs.
  JOURNAL   Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638
  PUBMED    21706014
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-06-26- 26"; volume: "8"; 
            issue: "8"; pages: "659-61"
COMMENT     Sequence Label: pLX303
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      21..156
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(176..195)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(177..195)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(205..222)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(205..221)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(214..236)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      363..818
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(450..469)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     660..681
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
       61 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
      121 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
      181 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
      241 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
      301 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
      361 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
      421 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      481 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      541 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      601 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      661 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      721 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
      781 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
      841 gtgcgcggaa
//
