LOCUS       sequence-66348-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral Gateway destination vector. Blasticidin 
            selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja 
            R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver 
            SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, 
            Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE
  TITLE     A public genome-scale lentiviral expression library of human ORFs.
  JOURNAL   Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638
  PUBMED    21706014
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-06-26- 26"; volume: "8"; 
            issue: "8"; pages: "659-61"
COMMENT     Sequence Label: pLX303
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(22..522)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(85..103)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(697..741)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gcttggccat ggtggtaccc cctggggaga gaggtcggtg attcggtcaa cgagggagcc
       61 gactgccgac gtgcgctccg gaggcttgca gaatgcggaa caccgcgcgg gcaggaacag
      121 ggcccacact accgccccac accccgcctc ccgcaccgcc ccttcccggc cgctgctctc
      181 ggcgcgccct gctgagcagc cgctattggc cacagcccat cgcggtcggc gcgctgccat
      241 tgctccctgg cgctgtccgt ctgcgagggt actagtgaga cgtgcggctt ccgtttgtca
      301 cgtccggcac gccgcgaacc gcaaggaacc ttcccgactt aggggcggag caggaagcgt
      361 cgccgggggg cccacaaggg tagcggcgaa gatccgggtg acgctgcgaa cggacgtgaa
      421 gaatgtgcga gacccagggt cggcgccgct gcgtttcccg gaaccacgcc cagagcagcc
      481 gcgtccctgc gcaaacccag ggctgccttg gaaaaggcgc aagggtcccc tcggggttgg
      541 gaggtgggtc tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag
      601 tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga ataattttat
      661 ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc
      721 caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat
      781 taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat
      841 ctgttccaga gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag
      901 ttt
//
