LOCUS       sequence-66360-e        9205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pAC-QSco
FEATURES             Location/Qualifiers
     source          1..9205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2187..2209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2206..2222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2277..4790
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     primer_bind     complement(8811..8828)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8811..8827)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8820..8842)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9036..9055)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9155..9177
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgaattctat attctaaaaa cacaaatgat acttctaaaa aaaaatcatg
     2281 aatggcatca actctgaatc aaatctttgc agatgcacct acttctcatt tccactgtca
     2341 catcattttt ccagatctcg ctgcctgtta tgtggcccac aaaccaagac acgttttatg
     2401 gccattaaag ctggctgatc gtcgccaaac accaaataca taatgaatat gtacacattc
     2461 gagaaagaag cgatcaaaga agcgtcttcg ggcggagtag gagaatgcgg aggagaagga
     2521 gaacgagctg atctagtatc tctccacaat ccaatgccaa ctgaccaact ggccatattc
     2581 ggagcaattt gaagccaatt tccatcgcct ggcgatcgct ccattcttgg ctatatgttt
     2641 ttcaccgtta cccggggcca ttttcaaaga ctcgtcggca agataagatt gtgtcactcg
     2701 ctgtctctct tcatttgtcg aagaatgctg aggaatttcg cgatgacgtc ggcgagtatt
     2761 ttgaagaatg agaataattt gtatttatac gaaaatcagt tagtggaatt ttctacaaaa
     2821 acatgttatc tatagataat tttgttgcaa aatatgttga ctatgacaaa gattgtatgt
     2881 atataccttt aatgtattct cattttctta tgtatttata atggcaatga tgatactgat
     2941 gatattttaa gatgatgcca gaccaaaagg cttgaatttc tgcgtctttt gccgaacgca
     3001 gtgcatgtgc aattgttgtt ttttggaata ttcaattttc ggactgtccg ctttgatttc
     3061 agtttcttgg cttattcaaa aagcaaagta aagccaaaaa agcgagatgg caataccaaa
     3121 tgcggcaaaa cggtagtgga aggaaagggg tgcggggcag cggaaggaag ggtggggcgg
     3181 ggcgtggcgg ggtctgtggc tgggcgcgac gtcaccgacg ttggagccac tcctttgacc
     3241 atgtgtgcgt gtgtgtatta ttcgtgtctc gccactcgcc ggttgttttt ttctttttat
     3301 gctgcgctct ctctagcgcc atctcgctta cgcatgctca acgcaccgca tgttgccgtt
     3361 tccttttatg cgtcattttg gctcgaaata ggcaattatt taaacaaaga ttagtcaacg
     3421 aaaacgctaa aataaataag tctacaatat ggttacttat tgccatgtgt gtgcagccaa
     3481 cgatagcaac aaaagcaaca acacaggtgg ctttccctct ttcacttttt gtttgcaagc
     3541 cgcgtgcgag caagacggca cgaccggcaa acgcaattac gctgacaaag agcagacgaa
     3601 gttttggcga aaaacatcaa ggcgcctgat acgaatgcat ttgcaataac aattgcgata
     3661 tttaatattg tttatgaagc tgtttgactt caaaacacac aaaaaaaaaa ataaaacaaa
     3721 ttatttgaaa gagaattagg aatcggacgc ttatcgttag ggtaacaaca agaaatgctt
     3781 actgagtcac agcctctgga aaactgccgc aagccagaga gagagagaaa aagagggaga
     3841 gcagcttaga ccgcatgtgc ttgtgtgtga ggcgtctctc tcttcgtctc tgttgcgcaa
     3901 acgcatagac tgcactgaaa aaatcgatta cctatttttt atgaatgaat atttgcacta
     3961 ttactattca aaactattaa gatagcaatc acattcaata gccaaatact ataccacctg
     4021 agcgatgcaa cgaaatgatc aatttgagca aaaatgctgc atatttagga cggcatcatt
     4081 atagaaatgc ttcttgctgt gtacttttct ctcgtctggc agctgtttcg ccgttattgt
     4141 taaaaccggc ttaagttagg tgtgttttct acgactagct agtgaatgcc ctactagaag
     4201 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     4261 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     4321 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     4381 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     4441 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     4501 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     4561 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     4621 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     4681 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     4741 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatccc
     4801 aacatgaaca ctattccagc gcgtcacgtt ggagatgtgg cagctcgtga cccactcccc
     4861 ctgccacata taagttccag tgtggccagt ggcatgaaac gatcgtttgc cactatggcc
     4921 atgctctata atgatacggg caatagcaat gatgtcggag cgcatgcgcg acgcccaccg
     4981 cgcactctga gtaacagccg cagcacatcg gctcaccggg tgccattggg tagctggagc
     5041 gccccgaact cgccaccgcg acgcgcactc ccacaccacc ccataacagc ttcgttcgac
     5101 ccggacgcct cgatcgtgat tgccggaatc cgcggtgctg gaaagtccac tctcgccata
     5161 atggcttcga cggcgatgaa gcgcaagatc gttgacctcg aatcggaatt tcaccacctg
     5221 actggcctgt cctccagcag ctacaagaaa acacacggcc cggtggacta tggtcgccgc
     5281 cagatagcaa tcctgcagaa catccttaac ctgcaccgaa cgcgcgccat tctggtatgt
     5341 tcctggttgg agcgcgatgt gcaggcaatg cttcaggatt tttcggtcag caacccggtg
     5401 atctatgtcc tcagggatgc caaagccatt gaggcccacc tgaaaggtta tgacaagtcg
     5461 aaggtgggta cgttgctgga tgcgacctcg accgtcctga ggcgatgcac gcgatttgag
     5521 ttcttcaatg tctccgaaga aaatctggat actcattccg ccagcacgtc ccccccagcg
     5581 gttccagatc agaggcatac tgctccctac ctgactctta agcgcgcaga gcgtcacttt
     5641 cttaagttcc tgtccttgat tttgcccaaa ggaaccatcc cgttcgtaga atccgccttc
     5701 ccactggcgt cggttccggt ggagcaacgc cgcttcacct atgcgctggc gcttcccgtg
     5761 tcggcactcc tggacaaggg agtcgacatt caggagcttg acgtaggcgt cgacgcaatc
     5821 gaaattatcg tagacgatct ggccacaagc gaatccggcc caactagccc cctcggcctc
     5881 gccccgcacc gagcaagcga aatctccagg gtggttggcg agatccgcag ggataccgtc
     5941 atacccatca tcctccacgt ggtcttcccg gagagggccc tgtacgaaga ggccctgttg
     6001 gccctgtaca tgacctacct gaaccacgcc ttgcgtctcg cgcccgatta ccttacagtg
     6061 gacctcggac tggatagcgg cctgctgggt cagttgacga cagtgcaggg cacaactaaa
     6121 gtgattggta acaagcagct ggctgaagtg aatagccctc gctggggcga cccatcgtgg
     6181 ttgcaggcct acgaaaaggc ccaaaatact ggctgtgacc ttgtgcggtt gacacgaccc
     6241 gcctcgaacc cgcgtgacaa tactgacatc cgtcagttcc atgttgccgt ggaggctgtg
     6301 ggtggcccac gactgccctt catcgcctat aatactggta ggctcggccg aaccagtatg
     6361 tgcttcaacg aaatcctgac gccagtcact cccgtcccca ccaaggagga tgccattggt
     6421 cttcgcaatc cggcacaccg ttacctgcag ccaccgttga ctgcactgga agcaacccag
     6481 gccctgtaca gcgcgtttgt tcacgaccca atgaagctgt acgttttcgg agctaatgtt
     6541 ggatacagcc tgagccccgc tatgcacaat gccgcgttga aagcctgcgg aatcccgcat
     6601 cattacaagc ccctgagtac tgctaacata ggcacccttc gtgaggtgat ctccgacccg
     6661 cagttcgcgg gagctagtgt aggtctgccc tttaaggtgg aaatcattag cctgactcac
     6721 tcgctgagcc gtcacgctaa agccattggc gcggtgaata cgttgattcc tgtgaggcat
     6781 cttacagcag atggaggcat ccccgacgag gtgtccatgt ttaataacat cagccaggca
     6841 ggtgctgtga gggcattgta cggcgagaat accgattgga tcggaatccg tgcctgtctg
     6901 cgtcgaggtt tgagtcctgc aaatgcagtc cgctccacat ccactggatt ggtgatcgga
     6961 gcaggcggca tggcccgagc agcggtgtac gcaatgctcc agctcggagt caagaagatc
     7021 ctcattttca accgcacctt cgctaacgcc gagaagctcg tgctgcattt cgagaatctg
     7081 ttggtcaggg atgccctgcc cctgttgtcg acaggccctc gcagccacga taacacgtgt
     7141 tttcacatca tacggtcgag ggatgatccg ctcccagaga acttcaagaa ccctaccatg
     7201 atagtgtcgt gcatcccaac gcacactgta gataatacgc ccgatcccga gttcaccgtg
     7261 ccattgcact ggttggacaa cccgaccggc ggaatcgtac tggagctgga ctacaagtgc
     7321 ttgacaagtc cactgttgga gcagacgcgg cgtgaggctc atcgcggatg ggttgcgatg
     7381 gatggcttgg acctgctccc tgaacaagga tttgcgcagt ttgaattgtt caccggccga
     7441 cgtgcaccgc gtcgcctgat gcgccgcgag gtcctgcgag catatccgga cgaccaggcc
     7501 aagagtcata ctgcacagct gcaacctcgt ctcaatggaa tagcaacgca gatttcgtaa
     7561 gcggccgcgt taaccgatcc gtcgaccatg aagatcaaga tcattgcccc gccagagcgc
     7621 aagtactctg tctggatcgg tggctccatc ctggcttcgc tgtccacctt ccagcagatg
     7681 tggatctcca agcaggagta cgacgagtcc ggcccctcca ttgtgcaccg caagtgcttc
     7741 taagaaggat cgcttgtctg ggcaagagga tcaggatcgg gatggtcttg attctgctgg
     7801 aggaggagga ggagaagtcg aggaagcagc agcgaaagtg caagtgcgag tggtggaagt
     7861 ttggagtgca gcacaacaaa atcaacaaca acaccaacta caagatgaaa agagcggaac
     7921 cacctgcaca ccatcatcac tatcatcatc gttttgggcg catgttgtgt ggttccagcg
     7981 tattaatata attaatttat tccacatgag atatgatatg atatactatg tattttttgt
     8041 ttttttttta tttgtaaacc tttaatataa caagaactac aaaaaatgaa aatgagcgaa
     8101 aatgcatatt ctgccattcc acacacacac caacaacacc caacacacgc acacccacaa
     8161 gcttacacac acacattcgc ggcatgacaa ggacatcaag ataaagaaga acttaaagaa
     8221 gatatttccc aaagcgcaaa aagaacacac acacattgca aaacacaaac aacacactag
     8281 cgttttgtac aattcgtcag caaccttatg tattattttt taattatgat gtaattataa
     8341 acaaagtgaa acaaaaatat gaaaacaaaa aggaaaatca aatctgtctt ctctttctcc
     8401 cgctctcctc gctctctgct gctaacctcg ccctctcctc tctcatcttt ttgtctgtct
     8461 ctcttccaca tttttgccgg ccggcaaaat aataacccac acacactcac acttggctgc
     8521 agtttcgcgt gcgatattca cacacattca agcatacaag catacataca tatgtatttt
     8581 ttttttattt gtacactttt ctaattgcat gcgtatcgat tgataagttt acgcctgaaa
     8641 atgttaatta aaatgtgaaa atgcaactga aaaactgatg aaatgaaaca acaacaagcg
     8701 aacaatttgc tacatgtgta ttgtctaaca accgttactg caacggttgc ttcggaaaag
     8761 ggtgaagagg aagagggcac gaggtcgacc tgcaggcatg caagcttggc actggccgtc
     8821 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     8881 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     8941 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
     9001 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     9061 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     9121 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     9181 tcaccgtcat caccgaaacg cgcga
//
