LOCUS       sequence-66361-e       12327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12327)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 12327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pCaSpeR-act5cB-QF#7
FEATURES             Location/Qualifiers
     source          1..12327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..586)
                     /label=P element 5' end
     gene            complement(597..4725)
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     CDS             complement(join(1364..1978,2049..2180,2401..2716,2778..3432,
                     3507..3780,4175..4246))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4698..4716
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     promoter        4846..7303
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5c promoter"
     misc_feature    join(9335..9536,9537..9567)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(10104..10123)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10223..10245
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10283..10301)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10369..10473
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10474..10542,10543..11334)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10692..10711)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11505..12093
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11994..12013
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
      541 aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttaattctag
      601 ttccagtgaa atccaagcat tttctaaatt aaatgtattc ttattattat agttgttatt
      661 tttgatatat ataaacaaca ctattatgcc caccattttt ttgagatgca tctacacaag
      721 gaacaaacac tggatgtcac tttcagttca aattgtaacg ctaatcactc cgaacaggtc
      781 acaaaaaatt accttaaaaa gtcataatat taaattagaa taaatatagc tgtgagggaa
      841 atatatacaa atatattgga gcaaataaat tgtacataca aatatttatt actaatttct
      901 attgagacga aatgaaccac tcggaaccat ttgagcgaac cgaatcgcgc ggaactaacg
      961 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     1021 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     1081 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     1141 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     1201 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     1261 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     1321 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     1381 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     1441 ccacgtagtc cagcggcaga tcggcggcgg agaagttaag cgtctccagg atgaccttgc
     1501 ccgaactggg gcacgtggtg ttcgacgatg tgcagctaat ttcgcccggc tccacgtccg
     1561 cccattggtt aatcagcaga ccctcgttgg cgtaacggaa ccatgagagg tacgacaacc
     1621 atttgaggta tactggcacc gagcccgagt tcaagaagaa gccgccaaag agcaggaatg
     1681 gtatgataac cggcggaccc acagacagcg ccatcgaggt cgaggagctg gcgcaggata
     1741 ttagatatcc gaaggacgtt gacacattgg ccaccagagt gaccagcgcc aggcagttga
     1801 agaagtgcag cactccggcc cgcagtccga tcatcggata ggcaatcgcc gtgaagacca
     1861 gtggcactgt gagaaaaagc ggtaattcgg caatcgtttt gcccagaaag tatgtgtcac
     1921 agcgataaag tcgacttcgg gcctccctca taaaaactgg cagctctgag gtgaacacct
     1981 aaatcgaatc gattcattag aaagttagta aattattaat atgcaaatgt attctaaaca
     2041 agacttacat ttatcgtggc aaagacgttt tgaaaggtca tgttggtcag gaagaggaag
     2101 atggctccgt tgatattcat cacgcccact tgcgtgagtt gttggcccaa aaagatgagg
     2161 ccaatcaaga tggcaaccat ctgcaaatta aaatgttact cgcatctcat taatattcat
     2221 atcttcaaca tgttcgcgag ttaaatgaaa tttatttatt ttctgcaaaa ctataaacta
     2281 tacatctcat tgaaaaaaac taagaagggt gtggaatcag gcaattctaa ctaaaatcta
     2341 gcgaatttgt ttccaagaat tgtaagcgtt atatcatttg tttccactgg aaccactcac
     2401 cgttgtctga ataagtcgca cttttacgag gagtggttcc ttgagcaccg acagccagga
     2461 tcgccacagg accgcccgga actgcatgaa ccaggtggcc ttgtaggtgt acccattctc
     2521 cggctgctcc agtggcttct ccaaattttt ggtggccaac aactgctcca tatcccgggc
     2581 tactttgcta atagcaaaat tgtcgcatat cttggcgatc cgatcacggg actcgatctc
     2641 ccgtccgggc acaacggcca acacctgtac gtaaaagtcc gccggattgt agttggtagg
     2701 acactgggca cccacgctgg ataggagttg agatgtaatg taatgctaga tacccttaat
     2761 aaacacatcg aactcactag gaaaagaagt cgacggcttc gctgggagtg cccaagaaag
     2821 ctaccctgcc ctcggccatc agaaggatct tgtcaaagag ctcaaacagc tcggaagacg
     2881 gctgatgaat ggtcaggatg acggtcttgc ccttctgcga cagcttcttc agcacctgga
     2941 cgacgctgtg ggcggtaaat gagtccagtc cggaggtggg ctcatcgcag atcagaagcg
     3001 gcggatcggt tagtgcctcg gaggcgaatg ccagacgctt cctttctccg ccggacagac
     3061 ctttcaccct gccgggcaca ccgatgatcg tgtgctgaca tttgctgagc gaaagctcct
     3121 ggatcacctg atccacgcgg gccactcgct gccgataggt cagatgtcgt ggcatccgca
     3181 ccatggcctg gaaaatcagg tgttccctgg ccgttaggga gccgataaag aggtcatcct
     3241 gctggacata ggcgcacctg gcctgcatct ccttggcgtc cacaggttgg ccattgagca
     3301 gtcgcatccc ggatggcgat acttggatgc cctgcggcga tcgaaaggca agggcattca
     3361 gcagggtcgt ctttccggca ccggaactgc ccatcacggc caaaagttcg cccggatagg
     3421 ccacgccgca aactgagttt caaattggta attggaccct ttattaagat ttcacacaga
     3481 tcagccgact gcgaatagaa actcaccgtt cttgagcaaa tgtttcctgg gcgccggtat
     3541 gtgtcgctcg ttgcagaata gtccgcgtgt ccggttgacc agctgccgcc atccggagcc
     3601 cggctgattg accgccccaa agatgtccat attgtgccag gcataggtga ggttctcggc
     3661 tagttggccg ctccctgaac cggagtcctc cggcggactg ggtggcagga gcgtgccgta
     3721 gtttttggcc tgcccgaagc cctggttaat gcagctctgc gaagccgctc cgctgtcacc
     3781 ctgcaatgat aggggatctc aaatatcaac tacaagcgtt atgctcatct aaccccgaac
     3841 aaaacgaagt atcctacgaa gtaggtttat acttttattt attttttgtg catctaggat
     3901 cagcttaaaa tatctggttg ttatattttt tgtaaaaaag aatgtagtcg aaaatgaatg
     3961 cctttagatg tcttgatcat gatatgatct taaaaattgt cttatatagc gagcacagct
     4021 accagaataa tctgtttcgt gtcactattt gtttgtgcga ttgcggtttg ggatttttgt
     4081 gggtcgcagt tctcacgccg cagacaattt gatgttgcaa tcgcagttcc tatagatcaa
     4141 gtgaacttaa gatgtatgca catgtactac tcacattgtt cagatgctcg gcagatgggt
     4201 gtttgctgcc tccgcgaatt aatagctcct gatcctcttg gcccattgcc gggatttttc
     4261 acactttccc ctgcttaccc acccaaaacc aatcaccacc ccaatcactc aaaaaacaaa
     4321 caaaaataag aagcgagagg agttttggca cagcactttg tgtttaattg atggcgtaaa
     4381 ccgcttggag cttcgtcacg aaaccgctga caaagtgcaa ctgaaggcgg acattgacgc
     4441 taggtaacgc tacaaacggt ggcgaaagag atagcggacg cagcggcgaa agagacggcg
     4501 atatttctgt ggacagagaa ggaggcaaac agcgctgact ttgagtggaa tgtcattttg
     4561 agtgagaggt aatcgaaaga acctggtaca tcaaataccc ttggatcgaa gtaaatttaa
     4621 aactgatcag ataagttcaa tgatatccag tgcagtaaaa aaaaaaaatg ttttttttat
     4681 ctactttccg caaaaatggg ttttattaac ttacatacat actagaattc tatattctaa
     4741 aaacacaaat gatacttcta aaaaaaaatc atgaatggca tcaactctga atcaaatctt
     4801 tgcagatgca cctacttctc atttccactg tcacatcatt tttccagatc tcgctgcctg
     4861 ttatgtggcc cacaaaccaa gacacgtttt atggccatta aagctggctg atcgtcgcca
     4921 aacaccaaat acataatgaa tatgtacaca ttcgagaaag aagcgatcaa agaagcgtct
     4981 tcgggcggag taggagaatg cggaggagaa ggagaacgag ctgatctagt atctctccac
     5041 aatccaatgc caactgacca actggccata ttcggagcaa tttgaagcca atttccatcg
     5101 cctggcgatc gctccattct tggctatatg tttttcaccg ttacccgggg ccattttcaa
     5161 agactcgtcg gcaagataag attgtgtcac tcgctgtctc tcttcatttg tcgaagaatg
     5221 ctgaggaatt tcgcgatgac gtcggcgagt attttgaaga atgagaataa tttgtattta
     5281 tacgaaaatc agttagtgga attttctaca aaaacatgtt atctatagat aattttgttg
     5341 caaaatatgt tgactatgac aaagattgta tgtatatacc tttaatgtat tctcattttc
     5401 ttatgtattt ataatggcaa tgatgatact gatgatattt taagatgatg ccagaccaca
     5461 ggctgatttc tgcgtctttt gccgaacgca gtgcatgtgc ggttgttgtt ttttggaata
     5521 gtttcaattt tcggactgtc cgctttgatt tcagtttctt ggcttattca aaaagcaaag
     5581 taaagccaaa aaagcgagat ggcaatacca aatgcggcaa aacggtagtg gaaggaaagg
     5641 ggtgcggggc agcggaagga agggtggggc ggggcgtggc ggggtctgtg gctgggcgcg
     5701 acgtcaccga cgttggagcc actcctttga ccatgtgtgc gtgtgtgtat tattcgtgtc
     5761 tcgccactcg ccggttgttt ttttcttttt atctcgctct ctctagcgcc atctcgtacg
     5821 catgctcaac gcaccgcatg ttgccgtgtc ctttatgcgt cattttggct cgaaataggc
     5881 aattatttaa acaaagatta gtcaacgaaa acgctaaaat aaataagtct acaatatggt
     5941 tacttattgc catgtgtgtg cagccaacga tagcaacaaa agcaacaaca cagtggcttt
     6001 ccctctttca ctttttgttt gcaagcgcgt gcgagcaaga cggcacgacc ggcaaacgca
     6061 attacgctga caaagagcag acgaagtttt ggccgaaaaa catcaaggcg cctgatacga
     6121 atgcatttgc aataacaatt gcgatattta atattgttta tgaagctgtt tgacttcaaa
     6181 acacacaaaa aaaaaaataa aacaaattat ttgaaagaga attaggaatc ggacagctta
     6241 tcgttacggg ctaacagcac accgagacga aatagcttac ctgacgtcac agcctctgga
     6301 agaactgccg ccaagcagac gatgcagagg acgacacata gagtagcgga gtaggccagc
     6361 gtagtacgca tgtgcttgtg tgtgaggcgt ctctctcttc gtctcctgtt tgcgcaaacg
     6421 catagactgc actgagaaaa tcgattacct attttttatg aatgaatatt tgcactatta
     6481 ctattcaaaa ctattaagat agcaatcaca ttcaatagcc aaatactata ccacctgagc
     6541 gatgcaacga aatgatcaat ttgagcaaaa atgctgcata tttaggacgg catcattata
     6601 gaaatgcttc ttgctgtgta cttttctctc gtctggcagc tgtttcgccg ttattgttaa
     6661 aaccggctta agttaggtgt gttttctacg actagtgaat gccctactag aagatgtgtg
     6721 ttgcacaaaa tgtccctgga ataaccaatt tgaagtgcag atagcagtaa acgtaagcta
     6781 atatgaatat tatttaactg taatgtttta atatcgctgg acattactaa taaacccact
     6841 ataaacacat gtacatatgt atgttttggc atacaatgag tagttgggga aaaaatgtgt
     6901 aaaagcaccg tgaccatcac agcataaaga taaccagctg aagtatcgaa tatgagtaac
     6961 ccccaaattg aatcacatgc cgcaactgat aggacccatg gaagtacact cttcatggcg
     7021 atatacaaga cacacacaag cacgaacacc cagttgcgga ggaaattctc cgtaaatgaa
     7081 aacccaatcg gcgaacaatt catacccata tatggtaaaa gttttgaacg cgacttgaga
     7141 gcggagagca ttgcggctga taaggtttta gcgctaagcg ggctttataa aacgggctgc
     7201 gggaccagtt ttcatatcac taccgtttga gttcttgtgc tgtgtggata ctcctcccga
     7261 cacaaagccg ctccatcagc cagcagtcgt ctaatccaga gaccccggat ccaacatgcc
     7321 acccaagcgc aaaacgctta acgctgcggc tgaggctaac gctcatgccg acggacacgc
     7381 cgacggaaac gccgacggac acgtggccaa tacggccgcg tcctcgaata atgcgaggtt
     7441 cgctgatctc actaacatcg atactccggg tctgggaccc acaactacga ccctgctcgt
     7501 ggaaccagca cgctcaaagc gtcaacgagt gtcccgcgca tgcgaccagt gccgtgcagc
     7561 ccgagagaaa tgcgacggaa tacagcctgc gtgtttcccg tgcgtttccc agggaaggtc
     7621 ctgcacttat caggcttcgc cgaaaaagag gggagttcaa accggttata ttcgtacgct
     7681 ggagctcgcc ctcgcctgga tgtttgaaaa tgtcgcgcgt tccgaagatg ccttgcataa
     7741 cctcctcgtc cgtgacgccg gacaaggatc agctctgctc gttggtaaag attcgccggc
     7801 tgccgagcga ctccatgccc gttgggctac tagccgtgtc aataagagca ttacccgcct
     7861 cctccgtcag ttggagctcc ctcctaccgc cacggctacg gcctcgataa tgccgcacgt
     7921 gatggagcag cctctcagta ccagcattaa ccccgtcaac gaccgcttca acggtattcc
     7981 caaccccact ccgtataact ccgatgcagc tctcgatgct atcactcaga ccaacgatta
     8041 tggaagcgta aatacacatg gtatcctctc tacttacccg ccaccggcta cgcaccttaa
     8101 tgaagcttcc gtcgctctcg ctcccggtgg cgcccccccc cgaccgcctc ctccgtatgt
     8161 tgacagcacg accaatcacc cgccgtacca ctcgaatctg gttccaatgg cgaactttgg
     8221 ttactcgacc gttgattacg atgccatggt tgacgatttg gctagcattg aatacacgga
     8281 cgctgtggat gtcgacccac agtttatgac caatctggga ttcgttcctg gatgtaactt
     8341 ctccgacatt aatacatacg aacagtgagc ggccgcgatc cgtcgaccat gaagatcaag
     8401 atcattgccc cgccagagcg caagtactct gtctggatcg gtggctccat cctggcttcg
     8461 ctgtccacct tccagcagat gtggatctcc aagcaggagt acgacgagtc cggcccctcc
     8521 attgtgcacc gcaagtgctt ctaagaagga tcgcttgtct gggcaagagg atcaggatcg
     8581 ggatggtctt gattctgctg gaggaggagg aggagaagtc gaggaagcag cagcgaaagt
     8641 gcaagtgcga gtggtggaag tttggagtgc agcacaacaa aatcaacaac aacaccaact
     8701 acaagatgaa aagagcggaa ccacctgcac accatcatca ctatcatcat cgttttgggc
     8761 gcatgttgtg tggttccagc gtattaatat aattaattta ttccacatga gatatgatat
     8821 gatatactat gtattttttg tttttttttt atttgtaaac ctttaatata acaagaacta
     8881 caaaaaagtg aaaatgagcg aaaatgcata ttctgccatt ccacacacac accaacaaca
     8941 cccaacacac gcacacccac aagcttacac acacacacat tcgcggcatg acaaggacat
     9001 caagataaag aagaacttaa agaagatatt tcccaaagcg caaaaagaac acacacacat
     9061 tgcaaaacac aaacaacaca ctagcgtttt gtacaattcg tcagcaacct tatgtattat
     9121 tttttaatta tgatgtaatt ataaacaaag tgaaacaaaa atatgaaaac aaaaaggaaa
     9181 atcaaatctg tcttctcttt ctcccgctct cctcgctctc tgctgctaac ctcgccctct
     9241 cctctctcat ctttttgtct gtctctcttc acatttttgc cggccggcaa aataataacc
     9301 cacacacact cacacttggc tgcagcccaa gctttgcgta ctcgcaaatt attaaaaata
     9361 aaactttaaa aataatttcg tctaattaat attatgagtt aattcaaacc ccacggacat
     9421 gctaagggtt aatcaacaat catatcgctg tctcactcag actcaatacg acactcagaa
     9481 tactattcct ttcactcgca cttattgcaa gcatacgtta agtggatgtc tcttgccgac
     9541 gggaccacct tatgttattt catcatggtc tggccattct catcgtgagc ttccgggtgc
     9601 tcgcatatct ggctctaaga cttcgggccc gacgcaagga gtagccgaca tatatccgaa
     9661 ataactgctt gttttttttt ttaccattat taccatcgtg tttactgttt attgccccct
     9721 caaaaagcta atgtaattat atttgtgcca ataaaaacaa gatatgacct atagaataca
     9781 agtatttccc cttcgaacat ccccacaagt agactttgga tttgtcttct aaccaaaaga
     9841 cttacacacc tgcatacctt acatcaaaaa ctcgtttatc gctacataaa acaccgggat
     9901 atatttttta tatacatact tttcaaatcg cgcgccctct tcataattca cctccaccac
     9961 accacgtttc gtagttgctc tttcgctgtc tcccacccgc tctccgcaac acattcacct
    10021 tttgttcgac gaccttggag cgactgtcgt tagttccgcg cgattcggtg cggtatttca
    10081 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
    10141 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
    10201 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
    10261 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
    10321 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
    10381 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
    10441 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
    10501 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
    10561 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
    10621 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
    10681 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
    10741 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
    10801 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
    10861 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
    10921 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
    10981 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
    11041 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
    11101 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
    11161 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
    11221 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
    11281 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
    11341 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    11401 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    11461 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    11521 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    11581 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    11641 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    11701 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    11761 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    11821 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    11881 tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    11941 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    12001 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    12061 tgatgctcgt caggggggcg gagcctatgg aaaaacgcct tcttcttgaa ctcgggctcg
    12121 gtgccagtat acctcaaatg gttgtcgtac ctctcatggt tccgttacgc caacgagggt
    12181 ctgctgatta accaatgggc ggacgtggag ccgggcgaaa ttagctgcac atcgtcgaac
    12241 accacgtgcc ccagttcggg caaggtcatc ctggagacgc ttaacttctc cgccgccgat
    12301 ctgccgctgg actacgtggg tctggcc
//
