LOCUS       sequence-66366-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Metzger T, Gache V, Xu M, Cadot B, Folker ES, Richardson BE, Gomes 
            ER, Baylies MK
  TITLE     MAP and kinesin-dependent nuclear positioning is required for 
            skeletal muscle function.
  JOURNAL   Nature. 2012 Mar 18;484(7392):120-4. doi: 10.1038/nature10914.
  PUBMED    22425998
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-18- 18"; volume: "484"; issue: "7392"; 
            pages: "120-4"
COMMENT     Sequence Label: pGEMT-Map7-856-1351
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(643..661)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(644..661)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(679..695)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(679..695)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(692..714)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    703..719
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(727..733,734..751,752..757))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    772..793
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctatagggcg aattgggccc gacgtcgcat gctcccggcc gccatggcgg ccgcgggaat
       61 tcgatttagg aactacaccg acagtcacag tggatgatgg tgcagggact ggggccgggt
      121 ccgtagctgg agcaggggct ggggttgcaa tgggggccga ggctggagca gcaggctcag
      181 gtggcttcac gggtgtcccc tcttcgactg tgacctcctc taccttcact aatggcaccc
      241 tgctctttac tgcgggtaaa gggtctttct tctcaggctc cactttcact tctctcttga
      301 caggccggat gttgccagga gaggaggagg gggcctgagc ggaagcagct ctgactgagc
      361 cgggaggcaa ggaggatgca ggccggggag taccaggcag atgaggcatg gacttggatg
      421 ggagccaaag gcgggctgga gccggtgacc tcgctttgag attagatgga gacagagtcc
      481 tgcgggcgcc cggggaaacg tggaagggaa cgtgttctct ttctcgctct cttttatggc
      541 tcgctagtcc atgaatggtc ataatcacta gtgaattcgc ggccgcctgc aggtcgacca
      601 tatgggagag ctcccaacgc gttggatgca tagcttgagt attctatagt gtcacctaaa
      661 tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
      721 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
      781 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
//
