LOCUS       sequence-66370-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Metzger T, Gache V, Xu M, Cadot B, Folker ES, Richardson BE, Gomes 
            ER, Baylies MK
  TITLE     MAP and kinesin-dependent nuclear positioning is required for 
            skeletal muscle function.
  JOURNAL   Nature. 2012 Mar 18;484(7392):120-4. doi: 10.1038/nature10914.
  PUBMED    22425998
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-18- 18"; volume: "484"; issue: "7392"; 
            pages: "120-4"
COMMENT     Sequence Label: pGEMT-Map7-1783-end
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(546..564)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(547..564)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(582..598)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(582..598)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(595..617)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    606..622
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(630..636,637..654,655..660))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    675..696
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tagggagaga ccgctgataa gaaaaccact gagcaaagaa atggtgacat agccaaagga
      121 gttctcactg gagagccaga agtacctgca ctgccgtgta tggcctcttc aggaaacgga
      181 gagtctgcag agagcccaca tggagtcgct ttacagcaat cagaagtgac cacagagagt
      241 tctccagatt tggaaaaaca gccaaatgaa aacggaatgt ccatacaaaa tgaaaatttt
      301 gaagaagtta taaacttacc tgttggatca aaagcgtcca gattagatgt caccaatgag
      361 aacccagaaa ttcctttgaa accaattttg gcctttaatg atgaagggac acttgggccc
      421 ctacctcagg tggatggtgt gcagacacaa cagaccgcag aagttaatca ctagtgaatt
      481 cgcggccgcc tgcaggtcga ccatatggga gagctcccaa cgcgttggat gcatagcttg
      541 agtattctat agtgtcacct aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg
      601 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      661 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      721 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      781 ggcggtttgc gtattgggcg ctcttccgct tcctcgct
//
