LOCUS       sequence-66376-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Marx CJ, Lidstrom ME
  TITLE     Development of improved versatile broad-host-range vectors for use 
            in methylotrophs and other Gram-negative bacteria.
  JOURNAL   Microbiology. 2001 Aug;147(Pt 8):2065-75.
  PUBMED    11495985
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology."; date: "2001-08"; volume: "147(Pt 8)"; pages: 
            "2065-75"
COMMENT     Sequence Label: pCM62
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..24
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     21..37
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     21..37
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    50..106
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(107..124)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(107..123)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(116..138)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(332..351)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     451..473
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(511..529)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcttgcatg
       61 cctgcaggtc gactctagag gatccccggg taccgagctc gaattcactg gccgtcgttt
      121 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      181 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      241 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
      301 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
      361 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
      421 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
      481 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
      541 atgtcatgat aataatggtt tcttagcacc ctttctcggt ccttcaacgt tcctgacaac
      601 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
      661 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
      721 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
      781 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
      841 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
      901 cgtgccggca tggatgc
//
