LOCUS       sequence-66387-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Shen MW, Fang F, Sandmeyer S, Da Silva NA
  TITLE     Development and characterization of a vector set with regulated 
            promoters for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):495-503. doi: 10.1002/yea.2930. Epub 2012 Nov
            19.
  PUBMED    23166051
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2930"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "495-503"
COMMENT     Sequence Label: pXP721
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(77..324)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     290..308
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(345..786)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(351..374)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    669..786
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 acttcgtata gcatacatta tacgaagtta tcccggggat cctctagagt cgacctgcag
       61 gcatgcaagc ttggccgcaa attaaagcct tcgagcgtcc caaaaccttc tcaagcaagg
      121 ttttcagtat aatgttacat gcgtacacgc gtctgtacag aaaaaaaaga aaaatttgaa
      181 atataaataa cgttcttaat actaacataa ctataaaaaa ataaataggg acctagactt
      241 caggttgtct aactccttcc ttttcggtta gagcggatgt ggggggaggg cgtgaatgta
      301 agcgtgacat aactaattac atgactcgag actagtggtt ttttctcctt gacgttaaag
      361 tatagaggta tattaacaat tttttgttga tacttttatt acatttgaat aagaagtaat
      421 acaaaccgaa aatgttgaaa gtattagtta aagtggttat gcagtttttg catttatata
      481 tctgttaata gatcaaaaat catcgcttcg ctgattaatt accccagaaa taaggctaaa
      541 aaactaatcg cattatcatc ctatggttgt taatttgatt cgttcatttg aaggtttgtg
      601 gggccaggtt actgccaatt tttcctcttc ataaccataa aagctagtat tgtagaatct
      661 ttattgttcg gagcagtgcg gcgcgaggca catctgcgtt tcaggaacgc gaccggtgaa
      721 gacgaggacg cacggaggag agtcttcctt cggagggctg tcacccgctc ggcggcttct
      781 aatccgtcat atgcctggtg cactctcagt acaatctgct ctgat
//
