LOCUS       sequence-66389-e         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Shen MW, Fang F, Sandmeyer S, Da Silva NA
  TITLE     Development and characterization of a vector set with regulated 
            promoters for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):495-503. doi: 10.1002/yea.2930. Epub 2012 Nov
            19.
  PUBMED    23166051
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2930"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "495-503"
COMMENT     Sequence Label: pXP722
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     101..124
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     terminator      151..398
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(167..185)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     protein_bind    complement(444..477)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        489..709
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 aatgcaaaaa ctgcataacc actttaacta atactttcaa cattttcggt ttgtattact
       61 tcttattcaa atgtaataaa agtatcaaca aaaaattgtt aatatacctc tatactttaa
      121 cgtcaaggag aaaaaaccac tagtctcgag tcatgtaatt agttatgtca cgcttacatt
      181 cacgccctcc ccccacatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct
      241 aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt atatttcaaa
      301 tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg aaaaccttgc
      361 ttgagaaggt tttgggacgc tcgaaggctt taatttgcgg ccaagcttgc atgcctgcag
      421 gtcgactcta gaggatcccc gggataactt cgtatagcat acattatacg aagttatgca
      481 ccacgctttt caattcaatt catcattttt tttttattct tttttttgat ttcggtttcc
      541 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      601 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
      661 cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc
      721 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
      781 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
      841 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
      901 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
      961 caatttttta ctcttcgaag ac
//
