LOCUS       sequence-66390-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Kabeya Y, Mizushima N, Ueno T, Yamamoto A, Kirisako T, Noda T, 
            Kominami E, Ohsumi Y, Yoshimori T
  TITLE     LC3, a mammalian homologue of yeast Apg8p, is localized in 
            autophagosome membranes after processing.
  JOURNAL   EMBO J. 2000 Nov 1. 19(21):5720-8.
  PUBMED    11060023
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2000 Nov 1. 19(21):5720-8."
COMMENT     Sequence Label: pCI-neo-myc-LC3-HA
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        33..51
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             80..109
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             116..145
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             152..181
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             197..619
                     /codon_start=1
                     /gene="Map1lc3b"
                     /product="rat LC3 protein"
                     /label=LC3
                     /note="Atg8 family member found on autophagosomes"
                     /translation="PSEKTFKQRRSFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPV
                     LDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESERDE
                     DGFLYMVYASQETFGTALAVTYMSALKATATGREPCL"
     CDS             635..724
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 cctccgttat tcgctcttaa ggctagagta cttaatacga ctcactatag gctagcctcg
       61 agaattcatg tctagaggtg aacaaaagtt gatttctgaa gaagatttga acggtgaaca
      121 aaagctaatc tccgaggaag acttgaacgg tgaacaaaaa ttaatctcag aagaagactt
      181 gaacggatcc tctagaccgt ccgagaagac cttcaaacag cgccggagct tcgaacaaag
      241 agtggaagat gtccggctca tccgggagca gcaccccacc aagatcccag tgattataga
      301 gcgatacaag ggtgagaagc agctgcccgt cctggacaag accaagttcc ttgtacctga
      361 tcacgtgaat atgagcgaac tcatcaagat aattagaagg cgcctgcagc tcaatgctaa
      421 ccaagccttc ttcctcctgg tgaatgggca cagcatggtg agtgtgtcca cacccatctc
      481 tgaagtgtac gagagcgaga gagatgaaga cggcttcctg tacatggtct atgcctccca
      541 ggagacgttc gggacagcac tggctgttac atacatgtca gctctgaagg caacagcaac
      601 aggaagagag ccatgcttgt ctagatctat cttttaccca tacgatgttc ctgactatgc
      661 gggctatccc tatgacgtcc cggactatgc aggatcctat ccatatgacg ttccagatta
      721 cgctgctcag tgcagatcta gatgagaatt cacgcgtggt acctctagct agagtcgacc
      781 cgggcggccg cttcccttta gtgagggtta atgcttcgag cagacatgat aagatacatt
      841 gatgagtttg gacacacata gatgatgaaa atg
//
