LOCUS       sequence-66391-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Kabeya Y, Mizushima N, Ueno T, Yamamoto A, Kirisako T, Noda T, 
            Kominami E, Ohsumi Y, Yoshimori T
  TITLE     LC3, a mammalian homologue of yeast Apg8p, is localized in 
            autophagosome membranes after processing.
  JOURNAL   EMBO J. 2000 Nov 1. 19(21):5720-8.
  PUBMED    11060023
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2000 Nov 1. 19(21):5720-8."
COMMENT     Sequence Label: pCI-neo-myc-LC3(G120A)-HA
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             83..112
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             119..148
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             155..184
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             200..622
                     /codon_start=1
                     /gene="Map1lc3b"
                     /product="rat LC3 protein"
                     /label=LC3
                     /note="Atg8 family member found on autophagosomes"
                     /translation="PSEKTFKQRRSFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPV
                     LDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESERDE
                     DGFLYMVYASQETFATALAVTYMSALKATATGREPCL"
     CDS             638..727
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     polyA_signal    833..954
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(844..863)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     898..917
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cctccagttc attcagctct taaggctaga gtacttaata cgactcacta taggctagcc
       61 tcgagaattc atgtctagag gtgaacaaaa gttgatttct gaagaagatt tgaacggtga
      121 acaaaagcta atctccgagg aagacttgaa cggtgaacaa aaattaatct cagaagaaga
      181 cttgaacgga tcctctagac cgtccgagaa gaccttcaaa cagcgccgga gcttcgaaca
      241 aagagtggaa gatgtccggc tcatccggga gcagcacccc accaagatcc cagtgattat
      301 agagcgatac aagggtgaga agcagctgcc cgtcctggac aagaccaagt tccttgtacc
      361 tgatcacgtg aatatgagcg aactcatcaa gataattaga aggcgcctgc agctcaatgc
      421 taaccaagcc ttcttcctcc tggtgaatgg gcacagcatg gtgagtgtgt ccacacccat
      481 ctctgaagtg tacgagagcg agagagatga agacggcttc ctgtacatgg tctatgcctc
      541 ccaggagacg ttcgcgacag cactggctgt tacatacatg tcagctctga aggcaacagc
      601 aacaggaaga gagccatgct tgtctagatc tatcttttac ccatacgatg ttcctgacta
      661 tgcgggctat ccctatgacg tcccggacta tgcaggatcc tatccatatg acgttccaga
      721 ttacgctgct cagtgcagat ctagatgaga attcacgcgt ggtacctcta gctagagtcg
      781 acccgggcgg ccgcttccct ttagtgaggg ttaatgcttc gagcagacat gataagatac
      841 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
      901 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaaccac
      961 a
//
