LOCUS       sequence-66392-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Kabeya Y, Mizushima N, Ueno T, Yamamoto A, Kirisako T, Noda T, 
            Kominami E, Ohsumi Y, Yoshimori T
  TITLE     LC3, a mammalian homologue of yeast Apg8p, is localized in 
            autophagosome membranes after processing.
  JOURNAL   EMBO J. 2000 Nov 1. 19(21):5720-8.
  PUBMED    11060023
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2000 Nov 1. 19(21):5720-8."
COMMENT     Sequence Label: pCI-neo-myc-LC3(deltaC22)
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             79..108
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             115..144
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             151..180
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    636..757
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(647..666)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     701..720
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctccgttatt cgctcttaag gctagagtac ttaatacgac tcactatagg ctagcctcga
       61 gaattcatgt ctagaggtga acaaaagttg atttctgaag aagatttgaa cggtgaacaa
      121 aagctaatct ccgaggaaga cttgaacggt gaacaaaaat taatctcaga agaagacttg
      181 aacggatcct ctagaccgtc cgagaagacc ttcaaacagc gccggagctt cgaacaaaga
      241 gtggaagatg tccggctcat ccgggagcag caccccacca agatcccagt gattatagag
      301 cgatacaagg gtgagaagca gctgcccgtc ctggacaaga ccaagttcct tgtacctgat
      361 cacgtgaata tgagcgaact catcaagata attagaaggc gcctgcagct caatgctaac
      421 caagccttct tcctcctggt gaatgggcac agcatggtga gtgtgtccac acccatctct
      481 gaagtgtacg agagcgagag agatgaagac ggcttcctgt acatggtcta tgcctcccag
      541 gagacgttcg ggtgagaatt cacgcgtggt acctctagag tcgacccggg cggccgcttc
      601 cctttagtga gggttaatgc ttcgagcaga catgataaga tacattgatg agtttggaca
      661 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
      721 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
      781 tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa
      841 atgtggtaaa tccgataagg atcgat
//
