LOCUS       sequence-66393-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Kabeya Y, Mizushima N, Ueno T, Yamamoto A, Kirisako T, Noda T, 
            Kominami E, Ohsumi Y, Yoshimori T
  TITLE     LC3, a mammalian homologue of yeast Apg8p, is localized in 
            autophagosome membranes after processing.
  JOURNAL   EMBO J. 2000 Nov 1. 19(21):5720-8.
  PUBMED    11060023
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2000 Nov 1. 19(21):5720-8."
COMMENT     Sequence Label: pCI-neo-myc-LC3(deltaC22, G120A)
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        33..51
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             80..109
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             116..145
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             152..181
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    637..758
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(648..667)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     702..721
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctccgttcat tcgctcttaa ggctagagta cttaatacga ctcactatag gctagcctcg
       61 agaattcatg tctagaggtg aacaaaagtt gatttctgaa gaagatttga acggtgaaca
      121 aaagctaatc tccgaggaag acttgaacgg tgaacaaaaa ttaatctcag aagaagactt
      181 gaacggatcc tctagaccgt ccgagaagac cttcaaacag cgccggagct tcgaacaaag
      241 agtggaagat gtccggctca tccgggagca gcaccccacc aagatcccag tgattataga
      301 gcgatacaag ggtgagaagc agctgcccgt cctggacaag accaagttcc ttgtacctga
      361 tcacgtgaat atgagcgaac tcatcaagat aattagaagg cgcctgcagc tcaatgctaa
      421 ccaagccttc ttcctcctgg tgaatgggca cagcatggtg agtgtgtcca cacccatctc
      481 tgaagtgtac gagagcgaga gagatgaaga cggcttcctg tacatggtct atgcctccca
      541 ggagacgttc gcgtgagaat tcacgcgtgg tacctctaga gtcgacccgg gcggccgctt
      601 ccctttagtg agggttaatg cttcgagcag acatgataag atacattgat gagtttggac
      661 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
      721 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      781 ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa aacctctacn
      841 aatgtggtaa aatccgat
//
