LOCUS       sequence-66397-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Shen MW, Fang F, Sandmeyer S, Da Silva NA
  TITLE     Development and characterization of a vector set with regulated 
            promoters for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):495-503. doi: 10.1002/yea.2930. Epub 2012 Nov
            19.
  PUBMED    23166051
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2930"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "495-503"
COMMENT     Sequence Label: pXP831
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(77..324)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     290..308
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(393..627)
                     /gene="S. cerevisiae CUP1"
                     /label=CUP1 promoter
                     /note="inducible metallothionein promoter, regulated by 
                     Cu2+"
     primer_bind     complement(665..684)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     784..806
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 acttcgtata gcatacatta tacgaagtta tcccggggat cctctagagt cgacctgcag
       61 gcatgcaagc ttggccgcaa attaaagcct tcgagcgtcc caaaaccttc tcaagcaagg
      121 ttttcagtat aatgttacat gcgtacacgc gtctgtacag aaaaaaaaga aaaatttgaa
      181 atataaataa cgttcttaat actaacataa ctataaaaaa ataaataggg acctagactt
      241 caggttgtct aactccttcc ttttcggtta gagcggatgt ggggggaggg cgtgaatgta
      301 agcgtgacat aactaattac atgactcgag cctaggttta tgtgatgatt gattgattga
      361 ttgtacagtt tgtttttctt aatatctatt tcgatgactt ctatatgata ttgcactaac
      421 aagaagatat tataatgcaa ttgatacaag acaaggagtt atttgcttct cttttatatg
      481 attctgacaa tccatattgc gttggtagtc ttttttgctg gaacggttca gcggaaaaga
      541 cgcatcgctc tttttgcttc tagaagaaat gccagcaaaa gaatctcttg acagtgactg
      601 acagcaaaaa tgtctttttc taactagtaa caaggctaag atcatatgcc tggtgcactc
      661 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
      721 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
      781 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
      841 agggcctcgt gatacgcc
//
