LOCUS       sequence-66399-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses hM4D (Gi) neuronal inhibitor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Armbruster BN, Li X, Pausch MH, Herlitze S, Roth BL
  TITLE     Evolving the lock to fit the key to create a family of G 
            protein-coupled receptors potently activated by an inert ligand.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):5163-8. Epub 2007 Mar 
            2.
  PUBMED    17360345
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2007-03-20"; volume: "104(12)"; pages: 
            "5163-8. Epub 2007 Mar 2"
COMMENT     Sequence Label: pcDNA5/FRT-HA-hM4D(Gi)
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             113..139
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gcgtttaaac ttaagcttgg taccgagctc ggatccacca tgtacccata
      121 cgatgttcca gattacgcta tggccaactt cacacctgtc aatggcagct cgggcaatca
      181 gtccgtgcgc ctggtcacgt catcatccca caatcgctat gagacggtgg aaatggtctt
      241 cattgccaca gtgacaggct ccctgagcct ggtgactgtc gtgggcaaca tcctggtgat
      301 gctgtccatc aaggtcaaca ggcagctgca gacagtcaac aactacttcc tcttcagcct
      361 ggcgtgtgct gatctcatca taggcgcctt ctccatgaac ctctacaccg tgtacatcat
      421 caagggctac tggcccctgg gcgccgtggt ctgcgacctg tggctggccc tggactgcgt
      481 ggtgagcaac gcctccgtca tgaaccttct catcatcagc tttgaccgct acttctgcgt
      541 caccaagcct ctcacctacc ctgcccggcg caccaccaag atggcaggcc tcatgattgc
      601 tgctgcctgg gtactgtcct tcgtgctctg ggcgcctgcc atcttgttct ggcagtttgt
      661 ggtgggtaag cggacggtgc ccgacaacca gtgcttcatc cagttcctgt ccaacccagc
      721 agtgaccttt ggcacagcca ttgctggctt ctacctgcct gtggtcatca tgacggtgct
      781 gtacatccac atctccctgg ccagtcgcag ccgagtccac aagcaccggc ccgagggccc
      841 gaaggagaag aaagccaaga cgctggcctt cctc
//
