LOCUS       sequence-66400-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Shen MW, Fang F, Sandmeyer S, Da Silva NA
  TITLE     Development and characterization of a vector set with regulated 
            promoters for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):495-503. doi: 10.1002/yea.2930. Epub 2012 Nov
            19.
  PUBMED    23166051
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2930"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "495-503"
COMMENT     Sequence Label: pXP841
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(621..640)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     740..762
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(800..818)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgactcgaga ctagttgtgt attacgatat agttaatagt tgatagttga ttgtatgctt
       61 tttgtagctt gatattctat ttaccaagaa gaaacaagaa gtgataaaaa caacaagaga
      121 gcagtagtaa gagtatttcg agtgtgaaaa aagtcgctac tggcactcta tttatatgtg
      181 ataggcatgc tatagcttta ccaaaaagtg aaccccattt ctatgctctc ctctgccttt
      241 tttttttttt tttttttcat tctctcaatc tgaaattctc ttatttctcc aacttataag
      301 ttggagatgc ccggtgttcc ggcagaggag atcagtctcg tgaagtggat ggtttcccgc
      361 ctgcgggcaa aacgtcataa catttttatg agcgaaagcc gttaatgaag acaaaatccc
      421 ttaattaaaa cattagaatg gtgattagaa aggcaggatt aatcagttac acaggctgta
      481 accggagaga cggatcataa ggcaattttt agataagact ggttagagtt cttggcatca
      541 gaaaatttga gaaacgattt ttccgtttgt ttgcccctac gttttgcggc cgctaaatca
      601 tatgcctggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
      661 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
      721 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
      781 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
      841 ataatggttt cttagacg
//
