LOCUS       sequence-66402-e         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rM3D (Gs) neuronal activator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Armbruster BN, Li X, Pausch MH, Herlitze S, Roth BL
  TITLE     Evolving the lock to fit the key to create a family of G 
            protein-coupled receptors potently activated by an inert ligand.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):5163-8. Epub 2007 Mar 
            2.
  PUBMED    17360345
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2007-03-20"; volume: "104(12)"; pages: 
            "5163-8. Epub 2007 Mar 2"
COMMENT     Sequence Label: pcDNA5/FRT-HA-rM3D(Gs)
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             197..223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gcgactctct ggctactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      121 cactagtcca gtgtggtgga attctgcaga taagctgctg caggtcgtcc tgggactatg
      181 tcagagagtc acaatgtacc cctacgacgt ccccgactac gccaccttgc acagtaacag
      241 tacaacctcg cctttgtttc ccaacatcag ctcttcctgg gtgcacagtc cctcggaggc
      301 agggctgccc ttggggacag tcactcagtt gggcagctac aacatttcac aagaaactgg
      361 gaatttctcc tcaaacgaca cctccagcga ccctctcggg ggtcacacca tctggcaagt
      421 ggtcttcatt gccttcttaa ctggcttcct ggcattggtg accatcattg gcaacatcct
      481 tgtcattgtg gccttcaagg tcaacaaaca gctgaagaca gtcaacaact acttcctctt
      541 aagcctggcc tgtgcagacc tgatcatcgg ggtcatttcc atgaacctgt tcactaccta
      601 catcattatg aaccgttggg cactggggaa cttagcctgc gacctctggc tctccattga
      661 ctgtgtggcc agcaatgcct ctgtcatgaa tctgctggtc atcagctttg acaggtactt
      721 ttccatcact tctccattcc gctaccagag cctgatgacc agggctcgag ctggtgtgat
      781 gattggtctg gcttgggtca tctcctttgt cctatgggct cctgccatct tgttctggca
      841 atactttgta gggaagagaa ctgtgccccc aggagaatgt ttcattcagt ttctgagtga
      901 gcccaccatc accttcggca cggcgatcgc tggcttttac atgcctgtca ccatcatgac
      961 tattttatac t
//
