LOCUS       sequence-66406-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Tschaharganeh DF, Chen X, Latzko P, Malz M, Gaida MM, Felix K, Ladu 
            S, Singer S, Pinna F, Gretz N, Sticht C, Tomasi ML, Delogu S, Evert 
            M, Fan B, Ribback S, Jiang L, Brozzetti S, Bergmann F, Dombrowski F,
            Schirmacher P, Calvisi DF, Breuhahn K
  TITLE     Yes-Associated Protein Up-regulates Jagged-1 and Activates the NOTCH
            Pathway in Human Hepatocellular Carcinoma.
  JOURNAL   Gastroenterology. 2013 Jun;144(7):1530-1542.e12. doi: 
            10.1053/j.gastro.2013.02.009. Epub 2013 Feb 16.
  PUBMED    23419361
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1053/j.gastro.2013.02.009"; journalName: "Gastroenterology"; 
            date: "2013-06"; volume: "144"; issue: "7"; pages: "1530-1542.e12"
COMMENT     Sequence Label: pT3-EFa1-HA-Jag1
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     67..84
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(122..163)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    216..240
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(267..356)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct
       61 ggcaactaga aggcacagtc gaggctgatc agcgggttta aactcattac taaccggtac
      121 gcgtagaatc gagaccgagg agagggttag ggataggctt accttcgaac cgcgggccct
      181 ctagactcga gcggccgcca ctgtgctgga tatcaaccac tttgtacaag aaagctgggt
      241 ctagatatct cgagtcagca ctgagcagcg taatctggaa cgtcatatgg ataggatcct
      301 gcatagtccg ggacgtcata gggatagccc gcatagtcag gaacatcgta tgggtaaaag
      361 atgcggccgc caccgcggat tacgatgtac tccatccggt tcaagctctg ggcactttcc
      421 aagtctctgt tgtcctgttt gtttgtccag ttcgggtgtt ttgtcggcgt gccgctgggg
      481 gccttctcct ctctgtctac cagcgtgtac acgggctgtt tggcaaaccg gactttctgc
      541 tggtgcttat ccatatcatc ctcttccact tccgagttgt gtgtcctgat ttttgacatt
      601 ttggagtttt tgttctcata atccttgatg gggactgtgt tggctccgtg tttctcgatg
      661 gggtttttga tttggttcag ctgttcgcgc acattgttgg tggtgttgtc ctcaggggca
      721 gagtgggtgt ggctgctggg cttccgccgc cgcttccgca cacaccagta gaaggctgtc
      781 acca
//
