LOCUS       sequence-66409-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Procko E, Hedman R, Hamilton K, Seetharaman J, Fleishman SJ, Su M, 
            Aramini J, Kornhaber G, Hunt JF, Tong L, Montelione GT, Baker D
  TITLE     Computational design of a protein-based enzyme inhibitor.
  JOURNAL   J Mol Biol. 2013 Sep 23;425(18):3563-75. doi: 
            10.1016/j.jmb.2013.06.035.
  PUBMED    23827138
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2013.06"; journalName: "J Mol Biol"; date: 
            "2013-09-23- 23"; volume: "425"; issue: "18"; pages: "3563-75"
COMMENT     Sequence Label: pETCON-HtsptLB12v.3
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(347..376)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(944..970)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(971..982)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atacgactca ctatagggcg aattggagct caattctctt aggattcgat tcacattcat
       61 ctttttttag ctactaatag gataaattat aggaatttat aacaaattga aatatggcag
      121 gcagcaaaat taaaattgtc ttagtttttt agtgtataga agtgaatagc tatataaagt
      181 atgtgtaaag ttggtaacgg aacgaaaaat agaaaaggat attacatggg aaaacatgtt
      241 gtttacggag aaatgaaaag tatattgtat tttgtacgag ctaaaagtac agtgggaaca
      301 aagtcgattt tgttacatct acactgttgt tatcagatct ctattacaag tcctcttcag
      361 aaataagctt ttgttcggat ccgcccccct cgagatcaga agcgatcttt ctttccaagt
      421 atctttttat actttcaacc atttggatgt catcttcagc aatggaatag tttgattctt
      481 cgacccaggc tgtcttatcg aattcaggtc tcatttcaat aaactttgcc aaaacgtatg
      541 gacagttcaa gtcgttttcg atatcaatga aaatgtgcca gtaagtttca atggaatata
      601 cggttctttc ttcgatttta ccgtctttat cgacatgtac ccatgctatt tcgaatctag
      661 catagtcttg gttcgttaaa acgtacaaac cgaactttat gtagttacca ggtaacaaca
      721 tgtaggctaa caaaccatat ctggttgagt gttcgaatgg ctttacgaac catctctttt
      781 taacagagaa taacaattca tgactcatat cctctgtata attaaaagac ttaacgacgt
      841 aatctacaac agacatttca ctcatttcgg aacccatatg gctagccgac cctccgcctc
      901 cgctaccgcc tccaccagag cctcctccac cactagcctg cagagcgtag tctggaacgt
      961 cgtatgggta tctaccttca atcgtcgagc tatt
//
