LOCUS       sequence-66414-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Procko E, Hedman R, Hamilton K, Seetharaman J, Fleishman SJ, Su M, 
            Aramini J, Kornhaber G, Hunt JF, Tong L, Montelione GT, Baker D
  TITLE     Computational design of a protein-based enzyme inhibitor.
  JOURNAL   J Mol Biol. 2013 Sep 23;425(18):3563-75. doi: 
            10.1016/j.jmb.2013.06.035.
  PUBMED    23827138
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2013.06"; journalName: "J Mol Biol"; date: 
            "2013-09-23- 23"; volume: "425"; issue: "18"; pages: "3563-75"
COMMENT     Sequence Label: pETCON-DnvLB16
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(346..375)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(820..846)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(847..858)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tacgactcac tatagggcga attggagctc aattctctta ggattcgatt cacattcatc
       61 tttttttagc tactaatagg ataaattata ggaatttata acaaattgaa atatggcagg
      121 cagcaaaatt aaaattgtct tagtttttta gtgtatagaa gtgaatagct atataaagta
      181 tgtgtaaagt tggtaacgga acgaaaaata gaaaaggata ttacatggga aaacatgttg
      241 tttacggaga aatgaaaagt atattgtatt ttgtacgagc taaaagtaca gtgggaacaa
      301 agtcgatttt gttacatcta cactgttgtt atcagatctc tattacaagt cctcttcaga
      361 aataagcttt tgttcggatc cgcccccctc gagtttgtaa cctatcaacc agtcaggctt
      421 ttcaggcaag gagatttcag taccactcaa tgtgtaatat tccttcaata cttccaagaa
      481 ttcttcccac aaaacgtcca aagcttcctt ccattgttcg aaatcttcct tggcgtagaa
      541 tgtccagttt gcagaaccag caattttgtc agccttgaat ttgaagtgca ttggcaaaac
      601 tctcaacaag ttgttaccag ccaaatgcca catagtttgg ataatgaatt cgatttcatc
      661 ttctaaggcg gtgtagtaaa cgtaagagga aaaggcttca atcaaaggga tgtaaaaggc
      721 ccacaatctc cattcgatgg ctaataatct catatggcta gccgaccctc cgcctccgct
      781 accgcctcca ccagagcctc ctccaccact agcctgcaga gcgtagtctg gaacgtcgta
      841 tgggtatcta ccttcaatcg tcgagctatt gt
//
