LOCUS       sequence-66415-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Procko E, Hedman R, Hamilton K, Seetharaman J, Fleishman SJ, Su M, 
            Aramini J, Kornhaber G, Hunt JF, Tong L, Montelione GT, Baker D
  TITLE     Computational design of a protein-based enzyme inhibitor.
  JOURNAL   J Mol Biol. 2013 Sep 23;425(18):3563-75. doi: 
            10.1016/j.jmb.2013.06.035.
  PUBMED    23827138
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2013.06"; journalName: "J Mol Biol"; date: 
            "2013-09-23- 23"; volume: "425"; issue: "18"; pages: "3563-75"
COMMENT     Sequence Label: pETCON-DnvLB16
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     100..123
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             455..466
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             467..493
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atgcaaaaac tgcataacca ctttaactaa tactttcaac attttcggtt tgtattactt
       61 cttattcaaa tgtaataaaa gtatcaacaa aaaattgtta atatacctct atactttaac
      121 gtcaaggaga aaaaaccccg gatcgaattc cctacttcat acattttcaa ttaagatgca
      181 gttacttcgc tgtttttcaa tattttctgt tattgcttca gttttagcac aggaactgac
      241 aactatatgc gagcaaatcc cctcaccaac tttagaatcg acgccgtact ctttgtcaac
      301 gactactatt ttggccaacg ggaaggcaat gcaaggagtt tttgaatatt acaaatcagt
      361 aacgtttgtc agtaattgcg gttctcaccc ctcaacaact agcaaaggca gccccataaa
      421 cacacagtat gtttttaagg acaatagctc gacgattgaa ggtagatacc catacgacgt
      481 tccagactac gctctgcagg ctagtggtgg aggaggctct ggtggaggcg gtagcggagg
      541 cggagggtcg gctagccata tgagattatt agccatcgaa tggagattgt gggcctttta
      601 catccctttg attgaagcct tttcctctta cgtttactac accgccttag aagatgaaat
      661 cgaattcatt atccaaacta tgtggcattt ggctggtaac aacttgttga gagttttgcc
      721 aatgcacttc aaattcaagg ctgacaaaat tgctggttct gcaaactgga cattctacgc
      781 caaggaagat ttcgaacaat ggaaggaagc tttggacgtt ttgtgggaag aattcttgga
      841 agtattgaag gaatattaca cattgagtgg tactgaaatc tccttgcctg aaaagcc
//
