LOCUS       sequence-66416-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Procko E, Hedman R, Hamilton K, Seetharaman J, Fleishman SJ, Su M, 
            Aramini J, Kornhaber G, Hunt JF, Tong L, Montelione GT, Baker D
  TITLE     Computational design of a protein-based enzyme inhibitor.
  JOURNAL   J Mol Biol. 2013 Sep 23;425(18):3563-75. doi: 
            10.1016/j.jmb.2013.06.035.
  PUBMED    23827138
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2013.06"; journalName: "J Mol Biol"; date: 
            "2013-09-23- 23"; volume: "425"; issue: "18"; pages: "3563-75"
COMMENT     Sequence Label: pET29b-HtsptLB12
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    75..99
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             114..136
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             633..650
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(703..721)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      717..764
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gccggcccga tgcgtccggc gtagaggatc gagatcgatc tcgatcccgc gaaattaata
       61 cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt
      121 aactttaaga aggagatata catatgggtt ccgaaatgag tgaaatgtct gttgtagatt
      181 acgtcgttac ttcttttaat tctacatcac aaatgagtca tgaattgtta ttctctgtta
      241 aaaagagatg gttcgtaaag ccattcgaac actcaagaag atatggtttg ttatcctaca
      301 agttgttacc tggtaactac ataaagttcg gtttgtacgt tttaaagaac caagactatg
      361 ctagattcga aatagcatgg gtacatgtcg ataaagacgg taaaatcgaa gaaagaaccg
      421 tatattccat tgaaacttac tggcacattt tcattgatat cgaaaacgac ttgaactgtc
      481 catacgtttt ggcaaagttt attgaaatga gacctgaatt cgaaaagaca gcctgggtcg
      541 aagaatcaaa ctattccatt gctgaagatg acatccaaat ggttgaaagt ataaaaagat
      601 acttggaaag aaagatcgct tctgatctcg agcaccacca ccaccaccac tgagatccgg
      661 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
      721 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
      781 tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cggggtgtgg
      841 taccgcacgt gacgct
//
