LOCUS       sequence-66418-e         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Procko E, Hedman R, Hamilton K, Seetharaman J, Fleishman SJ, Su M, 
            Aramini J, Kornhaber G, Hunt JF, Tong L, Montelione GT, Baker D
  TITLE     Computational design of a protein-based enzyme inhibitor.
  JOURNAL   J Mol Biol. 2013 Sep 23;425(18):3563-75. doi: 
            10.1016/j.jmb.2013.06.035.
  PUBMED    23827138
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2013.06"; journalName: "J Mol Biol"; date: 
            "2013-09-23- 23"; volume: "425"; issue: "18"; pages: "3563-75"
COMMENT     Sequence Label: pETCON-HtsptLB12
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     100..123
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             455..466
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             467..493
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atgcaaaaac tgcataacca ctttaactaa tactttcaac attttcggtt tgtattactt
       61 cttattcaaa tgtaataaaa gtatcaacaa aaaattgtta atatacctct atactttaac
      121 gtcaaggaga aaaaaccccg gatcgaattc cctacttcat acattttcaa ttaagatgca
      181 gttacttcgc tgtttttcaa tattttctgt tattgcttca gttttagcac aggaactgac
      241 aactatatgc gagcaaatcc cctcaccaac tttagaatcg acgccgtact ctttgtcaac
      301 gactactatt ttggccaacg ggaaggcaat gcaaggagtt tttgaatatt acaaatcagt
      361 aacgtttgtc agtaattgcg gttctcaccc ctcaacaact agcaaaggca gccccataaa
      421 cacacagtat gtttttaagg acaatagctc gacgattgaa ggtagatacc catacgacgt
      481 tccagactac gctctgcagg ctagtggtgg aggaggctct ggtggaggcg gtagcggagg
      541 cggagggtcg gctagccata tgggttccga aatgagtgaa atgtctgttg tagattacgt
      601 cgttacttct tttaattcta catcacaaat gagtcatgaa ttgttattct ctgttaaaaa
      661 gagatggttc gtaaagccat tcgaacactc aagaagatat ggtttgttat cctacaagtt
      721 gttacctggt aactacataa agttcggttt gtacgtttta aagaaccaag actatgctag
      781 attcgaaata gcatgggtac atgtcgataa agacggtaaa atcgaagaaa gaaccgtata
      841 ttccattgaa acttactggc acattttcat tgatatcgaa aacgacttga actgtccata
      901 cgttttggca aagtttattg aaatgagacc tgaattcgaa aagacagcct gggtcgaaga
      961 atcaaactat tccattgctg aaga
//
