LOCUS       sequence-66419-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Kowalska E, Ripperger JA, Muheim C, Maier B, Kurihara Y, Fox AH, 
            Kramer A, Brown SA
  TITLE     Distinct roles of DBHS family members in the circadian 
            transcriptional feedback loop.
  JOURNAL   Mol Cell Biol. 2012 Nov;32(22):4585-94. doi: 10.1128/MCB.00334-12. 
            Epub 2012 Sep 10.
  PUBMED    22966205
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00334-12"; journalName: "Mol Cell Biol"; date: 
            "2012-11"; volume: "32"; issue: "22"; pages: "4585-94"
COMMENT     Sequence Label: pG4-CMV-luc
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(120..323)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(149..169)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        324..627
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    716..810
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 ggcctttctt tatgtttttg gcgtcttcca tggtggcttt accaacagta ccggaatgcc
       61 aagcttgagc tcgagatctg agtccggtag cgctagcgga tctgacggtt cactaaacca
      121 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
      181 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
      241 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
      301 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc
      361 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
      421 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
      481 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
      541 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
      601 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
      661 cccgtaattg attactatta gggctagcac gcgtaagagc tcgctagagt ctccgctcgg
      721 aggacagtac tccgctcgga ggacagtact ccgctcggag gacagtactc cgctcggagg
      781 acagtactcc gctcggagga cagtactccg acctgcaggc atgcaagct
//
