LOCUS       sequence-66420-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Kowalska E, Ripperger JA, Muheim C, Maier B, Kurihara Y, Fox AH, 
            Kramer A, Brown SA
  TITLE     Distinct roles of DBHS family members in the circadian 
            transcriptional feedback loop.
  JOURNAL   Mol Cell Biol. 2012 Nov;32(22):4585-94. doi: 10.1128/MCB.00334-12. 
            Epub 2012 Sep 10.
  PUBMED    22966205
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00334-12"; journalName: "Mol Cell Biol"; date: 
            "2012-11"; volume: "32"; issue: "22"; pages: "4585-94"
COMMENT     Sequence Label: pG4-E6s-luc
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(66..262)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      80..215
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    435..529
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    561..655
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     misc_feature    675..766
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(707..726)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 gcctttcttt atgtttttgg cgtcttccat ggtggcttta ccaacagtac cggaatgcca
       61 agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
      121 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcg atggggcgga
      181 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
      241 gttgctgact aattgagatg cagatcgcag atcttccctg cacgtggact tgagggccca
      301 cgtgtcgtta cactgtcacg tggctaaacg cgtaagagat cgcagatctt ccctgcacgt
      361 ggacttgagg gcccacgtgt cgttacactg tcacgtggct aaacgcgtaa gctcgagctt
      421 gcatgcctgc aggtcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
      481 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagactcta
      541 gagcttgcat gcctgcaggt cggagtactg tcctccgagc ggagtactgt cctccgagcg
      601 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag
      661 actctagtat cgatagagaa atgttctggc acctgcactt gcactgggga cagcctattt
      721 tgctagtttg ttttgtttcg ttttgttttg atggagagcg tatgttagta ctatcgattc
      781 acacaaaaaa ccaacacaca gatgtaatga
//
