LOCUS       sequence-66424-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Kowalska E, Ripperger JA, Muheim C, Maier B, Kurihara Y, Fox AH, 
            Kramer A, Brown SA
  TITLE     Distinct roles of DBHS family members in the circadian 
            transcriptional feedback loop.
  JOURNAL   Mol Cell Biol. 2012 Nov;32(22):4585-94. doi: 10.1128/MCB.00334-12. 
            Epub 2012 Sep 10.
  PUBMED    22966205
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00334-12"; journalName: "Mol Cell Biol"; date: 
            "2012-11"; volume: "32"; issue: "22"; pages: "4585-94"
COMMENT     Sequence Label: pSCT-GAL93-DEST
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(81..87,88..105,106..111))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(136..260)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(591..610)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(592..610)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(638..737)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(667..687)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgacgg
       61 atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa agcctggggt
      121 gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
      181 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
      241 gcttttttgt acaaacttgt tgatcctgca ggggtaccca acaatgcttt tatatcctgt
      301 aaagaatcca ttttcaaaat catgtcaagg tcttctcgag gaaaaatcag tagaaatagc
      361 tgttccagtc tttctagcct tgattccact tctgtcagat gtgccctagt cagcggagac
      421 cttttggttt tgggagagta gcgacactcc cagttgttct tcagacactt ggcgcacttc
      481 ggtttttctt tggagcactt gagcttttta agtcggcaaa tatcgcatgc ttgttcgata
      541 gaagacagta gcttcatctt tcaggaggct tgcttcaagc ttagatccgt ccctatagtg
      601 agtcgtatta ccaggcgatc tgacggttca ctaaaccagc tctgcttata tagacctccc
      661 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
      721 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
      781 gaaatccccg tga
//
