LOCUS       sequence-66439-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Subauste MC, Von Herrath M, Benard V, Chamberlain CE, Chuang TH, Chu
            K, Bokoch GM, Hahn KM
  TITLE     Rho family proteins modulate rapid apoptosis induced by cytotoxic T 
            lymphocytes and Fas.
  JOURNAL   J Biol Chem. 2000 Mar 31. 275(13):9725-33.
  PUBMED    10734125
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Mar 31. 275(13):9725-33."
COMMENT     Sequence Label: pcDNA3-EGFP-Rac1-T17N
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(24..71,72..74,75..602)
                     /codon_start=1
                     /product="dominant negative mutant of human Rac family 
                     small GTPase 1"
                     /label=Rac1 (T17N)
                     /translation="MQAIKCVVVGDGAVGKNCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     promoter        complement(630..648)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(631..648)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(668..685)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    674..898
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tggacgagct gtacaaggaa ttcatgcagg ccatcaagtg tgtggtggtg ggagacggag
       61 ctgtaggtaa aaactgccta ctgatcagtt acacaaccaa tgcatttcct ggagaatata
      121 tccctactgt ctttgacaat tattctgcca atgttatggt agatggaaaa ccggtgaatc
      181 tgggcttatg ggatacagct ggacaagaag attatgacag attacgcccc ctatcctatc
      241 cgcaaacaga tgtgttctta atttgctttt cccttgtgag tcctgcatca tttgaaaatg
      301 tccgtgcaaa gtggtatcct gaggtgcggc accactgtcc caacactccc atcatcctag
      361 tgggaactaa acttgatctt agggatgata aagacacgat cgagaaactg aaggagaaga
      421 agctgactcc catcacctat ccgcagggtc tagccatggc taaggagatt ggtgctgtaa
      481 aatacctgga gtgctcggcg ctcacacagc gaggcctcaa gacagtgttt gacgaagcga
      541 tccgagcagt cctctgcccg cctcccgtga agaagaggaa gagaaaatgc ctgctgttgt
      601 aactcgagca tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc
      661 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
      721 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
      781 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
      841 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
      901 tctgaggcgg aa
//
