LOCUS       sequence-66451-e         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Mak AB, Ni Z, Hewel JA, Chen GI, Zhong G, Karamboulas K, Blakely K, 
            Smiley S, Marcon E, Roudeva D, Li J, Olsen JB, Punna T, Isserlin R, 
            Chetyrkin S, Gingras AC, Emili A, Greenblatt J, Moffat J
  TITLE     A lentiviral-based functional proteomics approach identifies 
            chromatin remodelling complexes important for the induction of 
            pluripotency.
  JOURNAL   Mol Cell Proteomics. 2010 May;9(5):811-23.
  PUBMED    20305087
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2010-05"; volume: "9(5)"; pages: "811-23"
COMMENT     Sequence Label: pLD-puro-2A-rtTA-TcVA
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             373..678
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     557..576
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gtactgcgat gagtggcagg gcggggcgta aagatctgga tccggcttac taaaagccag
       61 ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta
      121 tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac
      181 agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca
      241 caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg
      301 aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca
      361 ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg
      421 tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg
      481 gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc
      541 ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc
      601 ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
      661 atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtc
//
