LOCUS       sequence-66458-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Han C, Jan LY, Jan YN
  TITLE     Enhancer-driven membrane markers for analysis of nonautonomous 
            mechanisms reveal neuron-glia interactions in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jun 7;108(23):9673-8. doi: 
            10.1073/pnas.1106386108. Epub 2011 May 23.
  PUBMED    21606367
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1106386108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-06-7- 7"; volume: "108"; issue: "23"; pages: "9673-8"
COMMENT     Sequence Label: pDEST-HemmarG
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(121..834)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELY"
     primer_bind     complement(158..178)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     773..801
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(925..946)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aatctgaatt aaacattagt gttgcatgtt tcgaaggcgt ccgattgccg actggcttag
       61 ttaattaatt ctagattaga gggcaacttc attttcatag caaaagctgt ttgcgttctc
      121 gtagagttca tccatgccat gtgtaatccc agcagctgtt acaaactcaa gaaggaccat
      181 gtggtctctc ttttcgttgg gatctttcga aagggcagat tgtgtggaca ggtaatggtt
      241 gtctggtaaa aggacagggc catcgccaat tggagtattt tgttgataat ggtctgctag
      301 ttgaacgctt ccatcttcaa tgttgtgtct aattttgaag ttaactttga ttccattctt
      361 ttgtttgtct gccatgatgt atacattgtg tgagttatag ttgtattcca atttgtgtcc
      421 aagaatgttt ccatcttctt taaaatcaat accttttaac tcgattctat taacaagggt
      481 atcaccttca aacttgactt cagcacgtgt cttgtagttc ccgtcatctt tgaaaaatat
      541 agttctttcc tggacataac cttcgggcat ggcactcttg aaaaagtcat gctgtttcat
      601 atgatctggg tatcttgaaa agcattgaac accataagtt aaagtagtga caagtgttgg
      661 ccatggaaca ggtagttttc cagtagtgca aataaattta agggtaagtt ttccgtatgt
      721 tgcatcacct tcaccctctc cactgacaga aaatttgtgc ccattaacat caccatctaa
      781 ttcaacaaga attgggacaa ctccagtgaa aagttcttct cctttactgg ccatgttgtt
      841 gtcctcggag gaggcggttc cggagctgcc gctgccggtg ctgccggtgc catgccccat
      901 gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgc
//
